Amplified fragment length polymorphism

Evaluation of the genetic diversity of Bupleurum using amplified fragment length polymorphism analysis

S. Y. Ke, Shi, L. L., Ma, Y. Z., and Zhou, X. J., Evaluation of the genetic diversity of Bupleurum using amplified fragment length polymorphism analysis, vol. 14, pp. 2590-2599, 2015.

Radix bupleuri (Chaihu), the dried root of the Bupleurum plant, is an important component of traditional Chinese medicine. In this study, we examined the genetic diversity of 11 Bupleurum strains, originating from 7 provinces in China, using amplified fragment length polymorphism analysis. A total of 274 polymorphic bands were obtained using 6 primer combinations, indicating a high level of polymorphism across all strains.

Screening of molecular markers linked to dwarf trait in crape myrtle by bulked segregant analysis

Y. J. Ye, Liu, Y., Cai, M., He, D., Shen, J. S., Ju, Y. Q., Bian, X. M., Pan, H. T., and Zhang, Q. X., Screening of molecular markers linked to dwarf trait in crape myrtle by bulked segregant analysis, vol. 14, pp. 4369-4380, 2015.

Plant height is one of the most important traits of plant architecture as it modulates both economic and ornamental values. Crape myrtle (Lagerstroemia indica L.) is a popular ornamental woody plant because of its long-lasting mid-summer bloom, rich colors, and diversified plant architecture. These traits also make it an ideal model of woody species for genetic analysis of many ornamental traits.

Screening relevant genes of tolerance to low phosphorus in maize using cDNA-amplified fragment length polymorphism

H. Y. Jiang, Li, Z., Zhao, J., Ma, Q., Cheng, B. J., and Zhu, S. W., Screening relevant genes of tolerance to low phosphorus in maize using cDNA-amplified fragment length polymorphism, vol. 14, pp. 5731-5741, 2015.

Soil contains a large amount of phosphorus, but plants cannot absorb most of this phosphorus effectively. Low inorganic phosphorus has been singled out as a major constraint that leads to a perpetually low Zea mays (maize) grain yield. The fundamental approach to solving this problem is to screen new genes of low phosphorous (LP) tolerance. Consequently, the exploration and utilization of LP-tolerant genes are of great significance in plants. The maize inbred line 178 is an inbred LP-tolerant line.

Use of fluorescent-based amplified fragment length polymorphism to resolve phylogenetic relationships of Colchicum species from Turkey

K. Ö. Metin, Türktaş, M., Ertuğrul, F., and Kaya, E., Use of fluorescent-based amplified fragment length polymorphism to resolve phylogenetic relationships of Colchicum species from Turkey, vol. 13, pp. 1480-1490, 2014.

The study of phylogenetic relationships between 14 Colchicum taxa spread throughout Turkey was performed using a fluorescent-based amplified fragment length polymorphism (AFLP) technique. Five primer pair combinations were used in AFLP reactions. The data set was analyzed statistically using the NTSYS 2.1 software, and the neighbor-joining and maximum parsimony methods were implemented to generate phylogenetic trees. These analyses clustered the samples into 3 main clades. Both the neighbor-joining and maximum parsimony analyses resulted in similar topologies.

Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers

S. Shyamalamma, Chandra, S. B. C., Hegde, M., and Naryanswamy, P., Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers, vol. 7, pp. 645-656, 2008.

Artocarpus heterophyllus Lam., commonly called jackfruit, is a medium-sized evergreen tree that bears high yields of the largest known edible fruit. Yet, it has been little explored commercially due to wide variation in fruit quality. The genetic diversity and genetic relatedness of 50 jackfruit accessions were studied using amplified fragment length polymorphism markers. Of 16 primer pairs evaluated, eight were selected for screening of genotypes based on the number and quality of polymorphic fragments produced.

Molecular characterization of olive cultivars using amplified fragment length polymorphism markers

S. Ercisli, Barut, E., and Ipek, A., Molecular characterization of olive cultivars using amplified fragment length polymorphism markers, vol. 8, pp. 414-419, 2009.

We sampled six olive cultivars (Tavli Sati, Sati, Gorvela, Sacakli Otur, Butko, and Otur) from Coruh Valley, located in the northeast part of Turkey, and characterized them using amplified fragment length polymorphism (AFLP) markers. Some morphological and biochemical characteristics are also determined. Six AFLP primer combinations were used for molecular characterization and 66 AFLP markers were obtained. Six olive cultivars were classified into two major clusters using UPGMA clustering analysis; cv. Otur alone comprised the first group.

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