Table of Contents | Genet. Mol. Res. 2020 (3)
Annona sylvatica (Annonaceae) is a common medicinal plant used in folk medicine for fever and cough; it is found in several Brazilian states, including Mato Grosso do Sul. The local population uses A. sylvatica leaves for treating fever and cough; however, how this medicinal plant affects the patients is little understood. We evaluated the anti-inflammatory and anti-allergic properties of a methanolic extract of A. sylvatica (MEAS) leaves in mouse models of inflammation and allergy. The study employed male C57bL/6 mice for allergy models and male Swiss mice for the inflammation study. Oral treatments with MEAS (30 to 150 mg/kg) significantly inhibited the carrageenan-induced leukocyte migration and protein extravasation to the cavity in an air pouch model. In the allergic animal models, oral treatment with MEAS (150 mg/kg) significantly reduced histamine- and ovalbumin (OVA)-induced paw edema. Moreover, in the OVA-induced allergic lung inflammation model, oral treatment with MEAS (150 mg/kg) significantly inhibited neutrophil, eosinophil, and mononuclear cells migration to the lung. Pretreatment of neutrophils with MEAS (3, 150, 300 µg/mL) significantly reduced neutrophil chemotaxis induced by N-Formyl-Met-Leu-Ph (fMLP) and complement 5a (C5a, in a concentration-dependent manner. We conclude that A. sylvatica has anti-inflammatory and anti-allergic properties. Flavonoids and acetogenins, compounds found in the MEAS, could be responsible for these anti-inflammatory and anti-allergic effects.
Winter squash (Cucurbita moschata) has great importance as a food. Brazil has a wide genetic variability of squash; most of this is conserved in germplasm banks. The Vegetable Germplasm Bank of the Federal University of Viçosa (BGH-UFV) includes more than 350 accessions of squash; however, this germplasm is still little used. Characterization of accessions requires time, labor, and financial resources. Image-based, high-quality and large-scale phenotyping is a promising alternative tool. We propose digital phenotyping of C. moschata germplasm fruit. To achieve this, we evaluated 466 fruits from 148 accessions of squash from BGH-UFV and four checks. After longitudinal cutting, the fruits were evaluated on the basis of their length, diameter, and internal cavity dimensions. An image of every fruit was also obtained. Digital measurements were made using the software FENOM. The comparison between manual and digital forms of fruit evaluation was carried out with the software GENES. The comparisons were based on the analyses of simple linear regression, bias, the coefficient of Pearson correlation, the index of concordance, the index of performance, the efficiency of the method, the absolute average error, and the absolute maximum error. The evaluations based on images had high concordance (>0.93), almost perfect correlation (>0.99), and a performance classified as excellent (>0.92), in the evaluation of all the descriptors, when compared to manual measurements. We conclude that phenotyping of winter squash fruits based on digital images is promising for the characterization of C. moschata accessions, resulting in an efficient evaluation.
Genome size and chromosome number are basic biological characteristics that reveal a wide diversity in plants. The most widely used method to estimate genome size is flow cytometry; however, genomic estimation is only available for a small number of species. In this context, the DNA content of plants distributed in areas rich in species and endemism, such as the Brazilian Cerrado, remains little known. We examined genome size and number of chromosomes for the legume Dipteryx alata (popularly known as “baru”), a tree used for food, medicine, forage, recovery of degraded areas, landscaping and wood extraction. Dipteryx alata showed 2n = 16, with small chromosomes. Genome size or C-value was estimated at 1C = 0.825 pg which corresponds to 807.2 Megabases. This species is an important genetic resource, though it has a very small genome. In addition, this species is phylogenetically positioned in the first diverging lineage of Papilionoideae. Therefore, D. alata is a strong candidate among the tree species of the Brazilian Cerrado to be a model species in studies of comparative genomics of Leguminosae.
Chronic periodontal disease (CPD) is described as a recurrent inflammatory condition of tooth supporting tissues, caused by bacterial infections and self-destructive processes of immune activity mediated by pro-inflammatory cytokines, of which the interleukins 1β, 6 and 8 stand out; together with other biomolecules these cytokines mediate innate immunity. These molecules may have variations in their genes, such as single nucleotide polymorphisms, which together with environmental factors, can affect susceptibility to this disease condition. We investigated how polymorphisms of the IL1B, IL6 and CXCL8 genes, as well as sociodemographic and lifestyle aspects, affect susceptibility to chronic periodontal disease in a group of volunteers in Goiânia city. Peripheral blood samples from 152 volunteers were obtained via venipuncture and grouped into controls and a case group (CPD), according to the result of the periogram, previously elaborated, together with a questionnaire concerning habits and life style. The DNA of the samples was genotyped using PCR-RFLP and qPCR, for each molecular marker. A significant trend to develop CPD was detected for individuals who brush their teeth less than twice a day. The allele of minor frequency of the variants rs1143634, rs1800796, and rs2227539 were associated with CPD, revealing a significant predictive effect (P < 0.05). The most significant findings involved the T (rs1143634) and C (rs1800796) alleles, which were associated with increased risk, when examined individually and together. We concluded that this is a multifactorial disease, although the the genetic influence was predominant.
In Brazil, most sheep are of the Santa Inês breed, which shows rusticity in breeding and grazing systems, being able to offer better adaptation characteristics for animals with meat production ability, such as the Texel and Dorper sheep. They can also improve meat production in animals that have better characteristics for milk production, such as Lacaune and East Friesian breeds. We investigated the polymorphisms of calpastatin (CAST) and myostatin (GDF8) genes and their association with meat quality parameters of 84 lambs, including: 14 purebred Santa Inês and 70 crossbred animals of five genetic groups: 16 animals (½ Santa Inês x ½ Black Dorper); 12 animals (½ Santa Inês x ½ White Dorper); 19 animals (½ Santa Inês x ½ Texel); 14 animals (½ Santa Inês x ½ Lacaune); and 9 animals (½ Santa Inês x ½ East Friesian). To evaluate the polymorphism of the genes we used PCR and Simple Chain Conformation Polymorphism (SSCP) with DNA sequencing to determine where base exchange occurred in the gene sequences. The PCR-SSCP technique detected four bands of the CAST gene and three of the GDF-8 gene. In meat quality of sheep there was only one different breed group by Shear force with less value than Santa Ines breed group. The CAST gene had four genotypes with two single nucleotide polymorphisms (SNPs) (c.679A> G; c.383A> G), which resulted in the exchange of glutamic acid by glycine and threonine by alanine, which impacted pH values. Reduced pH values for BB and AB genotypes, and increased pH value in the AA genotype were found. Three genotypes of the DGF-8 gene with three SNPs were identified with changes of A-T, A-G, and T-G, influencing color hue angle (h*). After 10 days post mortem, there was an increase in hue angle the highest values were found for genotypes DF and DE. The polymorphisms of the CAST gene and in the promoter region of the GDF-8 gene influence meat color and final pH, affecting meat quality in various of the sheep breed groups.
One of the main global causes of mortality is cerebral ischemia. Studies report that alcohol abuse causes health problems, aggravating the conditions of neurological diseases. The role of microRNAs has been highlighted in the literature as biomarkers in various types of diseases, including neurological disorders. Evidence of dysregulation of serum miRNAs related to apoptosis in experimental brain ischemia associated with alcoholism is scarce. We evaluated the gene expression of miRNA-15b and 16 (apoptotic) and miRNA-21, -221 and -222 (anti-apoptotic) in the blood after experimental induction of temporary focal ischemia (90 minutes) by occlusion of the middle cerebral artery in rats, followed by reperfusion for 48 hours, associated with a chronic alcoholism model. Fifty-six rats were randomly divided into seven experimental groups: Control (C); Sham (S); Ischemic (I); Ischemia-reperfusion (IR), Alcoholic (A); Alcoholic and ischemic (I + A); Ischemia-reperfusion and Alcoholic (IR+A). The blood samples were collected for gene expression analysis of some serum miRNAs by PCR in real time. The serum expression of miRNA-16 was higher in the IR group compared to C and S groups (P < 0.05) but no association with chronic alcoholism was found. The serum expressions of miRNA-21, -221 and -222 were low in all groups and were not correlated with ischemic injury and/or chronic alcoholism. The serum expressions of miRNA-15b, -21, -221 and -222 were similar among the experimental groups, with no correlation with ischemia, with or without reperfusion, and/or alcoholism. The overexpression of miRNA-16 in the blood of I and IR groups demonstrated a correlation with the ischemic process, mainly after reperfusion for 48 hours, associated or not with alcoholism.
Myrica rubra has been cultivated for more than 2000 years it is one of most popular fruits in south China. We compared three M. rubra cultivars, "Muye" (MY), "DongKui" (DK), and "Zao Jia" (ZJ) to determine the causes of the differences in fruit color. We found changes in the anthocyanin and carotenoid contents during the fruit coloring and maturity phases. The anthocyanin contents of the three cultivars increased from the initial fruit coloring phase to the maturity phase. During this period, the total contents of anthocyanin and cyanidin in MY were higher than those in ZJ by 8.57 and 26.45% and higher than those in DK by 80.16 and 129.37%, respectively. RNA-Seq based transcriptome analysis of veraison and mature berries of the three M. rubra cultivars was conducted. The total numbers of genes and N50 lengths were 33,033 and 1,426 for MY, 35,166 and 1,145 for DK, and 31,374 and 476 for ZJ, respectively From the initial color-turning phase to the maturity phase, there were three up-regulated and three down-regulated genes in MY, while there were 3,058 up-regulated and 65 down-regulated genes in DK and 1,484 up-regulated and 1,169 down-regulated genes in ZJ. Based on functional insight of the differentially expressed genes, 3-Amino-1,2,4-triazole (3AT) would be the key gene responsible for color variation in these three cultivars. We concluded that the 3AT gene controls M. rubra berry color. Based on qRT-PCR, we found that expression of 3AT was much higher in MY and ZJ. This result was consistent with our RNA-Seq analysis. In conclusion, cy-3glu and peonidin are pigments that have a significant effect on the color of M. rubra fruit, and 3AT is a functional gene that regulates these pigments. These results could have practical significance for M. rubra selection strategies.
Understanding the genetic control of internode length is essential to develop more compact winter squash genotypes. Our objective was to elucidate the genetic control of internode length before and after emergence of the first female flower in winter squash, Cucurbita moschata. This was done by estimating the linear and quadratic genetic components and using the maximum likelihood estimation function. The parents used were the long-vined accession BGH 7319 (P1) and the compact cultivar ‘Tronco Verde’ (P2). The F1 plants from this cross were self-fertilized to obtain the F2 generation, and then they were backcrossed with P1 and P2 to obtain generations BC1 and BC2, respectively. By examining the linear and quadratic genetic components of variations in internode length, we found evidence of dominance effects both before and after flowering, with a reversal in dominance after flowering. Using maximum likelihood, we observed that the internode length before flowering was controlled by one major gene with additive and dominance effects, while the internode length after flowering was controlled by multiple genes with additive and dominance effects, plus environmental effects. Based on these results, strategies using backcrosses for introgression of the major gene controlling this trait before flowering and recurrent selection for introgression of the polygenes involved in trait control after flowering are recommended.
Rice (Oryza sativa) is crop that adapts well to diverse soil and climate conditions; breeding programs have generally been committed to identifying and selecting genotypes that are stable and have high productivity in various environments. In this sense, studies of adaptability and stability are of paramount importance to aid in the recommendation of cultivars, since it allows growers to obtain detailed information about the behavior of the genotypes in each region. We evaluatde the adaptability and stability of irrigated rice genotypes grown with continuous flooding, for the selection and recommendation of cultivars for crops or breeding programs. Eighteen genotypes were evaluated for grain yield in four agricultural years at three sites, covering 12 environments. The adaptability and stability were assessed by the methods of Eberhart and Russell, multiple centroids and GGE biplot. Genotypes behaved differently regarding stability and adaptability in the different environments. Both methodologies identified BRA 02691 and MGI 0607-1 as promising to be released as cultivars; however, classification inconsistencies occurred, such as for the line BRA 031001. Multiple centroid and GGE biplot methods demonstrated greater sensitivity than the Eberhart and Russell method. Using the methods simultaneously provides an innovative approach to the interpretation of GxE interactions and is a viable alternative for genotype classification. The genotype MGI 0607-1 showed promising behavior independent of the methodology used.
We compared three Pi-efficient (ATF-14B, ATF-53B, 101B) and four Pi-inefficient (ATF-16B, 116R, 136B, 187R) sorghum genotypes under different Pi concentrations. There were no significant differences between the groups in Pi-use efficiency under Pi-deprivation for anthocyanin accumulation, dry-weight matter, acid phosphatase activity (APA), and aerenchyma formation. However, both groups showed anthocyanin accumulation under Pi-deprivation. Under Pi-deficiency, there was a significant reduction of dry weight in both groups, with no significant differences between contrasting genotypes. All genotypes exhibited a significant increase in root/shoot ratios during Pi-deficiency, and these changes were not related to Pi-use efficiency. The total Pi content in roots and shoots in all genotypes was similar and represented less than 0.2 % of the total dry weight. For all genotypes, the Pi content in P+ treatment resulted in a significant variation ranging from 0.45 to 0.85% and 0.41 to 0.66% in roots and shoots, respectively. The genotype 187R had the highest P content in roots and shoots. APA activity showed increased activity only in the roots of both groups. The development of aerenchyma was conspicuous in the basal and in the middle root sections of all genotypes grown under different Pi levels. Two sorghum Pi-transporter genes were strongly overexpressed in the middle part of Pi-deprived roots of 136B genotype. We did not find differences that explain the Pi-use efficiency between efficient and inefficient genotypes. More studies are needed to elucidate the complex mechanism of P-utilization by sorghum plants.
Pyemotidae mites are ectoparasites of a large number of arthropods, and Pyemotes tritici (Acari: Pyemotidae) is often found parasitizing insects in various habitats, including in the laboratory. Here we report widespread infestation of P. tritici in laboratory colonies of various species of stored-products insects. P. tritici infestations were observed in laboratory colonies (MIPGrains/UFV) of Sitophilus zeamais (Coleoptera: Curculionidae), Alphitobius diaperinus (Coleoptera: Tenebrionidae), Lasioderma serricorne (Coleoptera: Anobiidae), Acanthoscelides obtectus (Coleoptera: Bruchidae), Rhyzopertha dominica (Coleoptera: Bostrichidae), Ephestia sp. (Lepidoptera: Pyralidae), and Tribolium castaneum (Coleoptera: Tenebrionidae). The widespread infestation of P. tritici in laboratory colonies of these species is a serious problem. This mite can substantially reduce insect populations or even kill them, making it impossible to rear the insects, as observed in this research. This ectoparasite is not recommended as a biological control agent in integrated pest management of stored-product pests because P. tritici can attack bees, mammals, and other animals and cause dermatitis in humans. In consideration of the above, the widespread infestation of P. tritici in colonies of S. zeamais, A. diaperinus, L. serricorne, A. obtectus, R. dominica, Ephestia sp., and T. castaneum indicates that this mite should be monitored when raising stored-products insects to ensure the development of the insect populations and ensure that the laboratory environment is safe.
Xanthomonas oryzae pv oryzae (Xoo) is a bacterial pathovar that causes a serious bacterial leaf blight disease of rice. This disease poses significant constraint on food security in Asia, as it causes yield loss in rice. There is an urgent need to control bacterial blight disease through resistance cultivars. However, the genetic potential of Malaysian rice cultivars has not been explored. We screened 10 cultivated Malaysian varieties with high yield performance for resistance genes using three simple sequence repeat and two sequence tagged sites markers coupled with phenotypic screening. All 10 rice genotypes were found to carry xa recessive gene. Four genotypes had two resistance genes tightly linked with the specific markers; Mahsuri Mutant carried the dominant resistance genes, xa4 and xa2 whereas NMR152 and the Tongkat Ali mutant had the dominant genes, xa21 and xa2. However, xa13 and xa5 resistance genes were not detected in this Malaysia rice germplasm group. In a greenhouse assessment, genotypes carrying more than a single resistance gene were found to be resistant against Xoo MXO 1410 isolates. These cultivars have potential as genetic materials for rice quality breeding programs.
The culture of summer squash (Cucurbita pepo) has great socioeconomic importance worldwide. Characterization of C. pepo germplasm has been predominantly performed by field evaluations, which is very time consuming. Thus, the validation of new techniques capable of optimizing time for the field germplasm selection process would be useful. We evaluated agronomic potential and genetic dissimilarity of C. pepo germplasm and gathered data to determine whether aerial images obtained by drone imaging could assist in the selection of vegetative vigor; this is the first such analysis for this crop. Sixty-five genotypes belonging to the vegetable germplasm bank of the Federal University of Uberlândia were evaluated, with three replications in a randomized block design. The variables evaluated were: production per plant, number of fruits per plant, leaf temperature, precocity, and the indexes SPAD (Soil Plant Analysis Development), LAI (Leaf Area Index), NDVI (Normalized Difference Vegetation Index) and NDRE (Normalized Difference Red Edge Index) the last three variables were obtained using drone imaging. Genetic divergence analysis was performed with multivariate techniques using generalized Mahalanobis distance and UPGMA clustering. Hybrid performance was compared by the Scott-Knott test. UPGMA clustering showed considerable genetic diversity, with the formation of 12 distinct groups. The largest relative contribution was from the leaf area index in the discrimination of the genotypes, demonstrating high efficiency in the validation of the image phenotyping technique. Eight genotypes stood out for yield, fruit number, precocity and high leaf area index, NDVI and NDRE values. The use of image phenotyping using NDVI and NDRE sensors was efficient to identify C. pepo genotypes that differed in plant vigor.
The cultivation of onion in Brazil has a relevant socioeconomic contribution. Despite the potential, there are few onion breeding programs for tropical conditions. One of the major obstacles is related to the complexity during the selection steps, since in onion, quantitative characters and low heritability predominate. For this reason, the purpose of this study was to assess the dynamics of expression between onion line traits based on phenotypic and genotypic correlations. Four sets of onion genotypes were assessed, comprising both advanced and segregating lines. The traits assessed were plant vigor, diameter of pseudostem, plant architecture, plant cycle, average bulb mass, bulb firmness and bulb postharvest. Phenotypic and genotypic correlations were calculated. Path analysis provided the estimate of the direct and indirect effects based on average bulb mass as the main variable. There was dynamics in trait expression. Coherence was reported for the correlation values of the different traits among the different germplasms (experiment 1 with 109 advanced lines; experiment 2 with 227 segregating lines; experiment 3 with 53 advanced lines; and experiment 4 with110 segregating lines). Correlations were positive for all average bulb mass x plant vigor experiments; plant architecture x plant vigor was also positive in all experiments, except for experiment 1 (109 advanced lines). The correlations which were larger in magnitude were those between bulb postharvest and plant cycle, and between bulb postharvest and average bulb mass. The phenotypic and genotypic correlations showed low association between traits in all experiments, except for experiment 3 (53 advanced lines), in which some pairs of correlations were moderate to high; however, the path analysis confirmed that the cycle (in days) and bulb postharvest showed weak cause-and-effect relationship with the main variable, rendering indirect selection impracticable for onion average bulb mass.
Bovine brucellosis is a zoonotic disease that has considerable economic impact on the market of products such as meat and milk. As Brazil has one of the largest cattle herds in the world, this disease is a significant agricultural and public health concern. In 2001, Brazil launched the National Program of Control and Eradication of Animal Brucellosis and Tuberculosis. This program included the vaccination of 3-8 month old calves using the smooth and attenuated S19 strain, as well as monitoring with serological tests. There have been reports of excretion and persistence of S19. In this context, the objective of this study was to monitor the persistence of the S19 vaccine strain in blood samples from vaccinated calves. Seroconversion was investigated to evaluate the vaccine immunogenicity. PCR assays of blood and serum were run at at: "day zero" (before vaccination), 1 to 15 days after vaccination and every month until 12 months, totaling 28 collections for each of 10 calves. The vaccine strain was detected throughout the study, even at day zero, before the calves were vaccinated. Sequencing analysis confirmed the presence of the S19 strain. Results from serological tests did not show agglutination in “day zero” samples, meaning the immune response was negative. An immune response was first detected in two samples on the fourth day. One hypothesis that could explain this positivity before vaccination is that the already vaccinated mothers could have transmitted the vaccine to the calves, as the cows presented excretion of the S19 strain in milk and/or urine. This is the first report of the finding of this bovine brucellosis vaccine strain in the blood of calves before vaccination.