ASSOCIATION OF RARRES2 CHEMERIN GENE POLYMORPHISM WITH TYPE 2 DIABETES MELLITUS AND METABOLIC SYNDROME: A CASE-CONTROL STUDY

Abstract

Background: Chemerin is type of adipokine that involved in metabolism, inflammation and glucose homeostasis of adipose tissues. Genetic variations in the RARRES2 gene may come up with metabolic abnormalities of type 2 diabetes mellitus (T2DM) in association with metabolic syndrome (MetS). The present study investigated the association of gene polymorphism of RARRES2 rs17171368 gene with T2DM with and without MetS and evaluated its relationship with selected metabolic parameters. Methods: A case-control study was conducted among 294 participants aged 25–75 years, comprising 98 patients with T2DM with MetS, 98 patients with T2DM without MetS and 98 healthy controls. Anthropometric, clinical and biochemical parameters were assessed. Serum chemerin levels were estimated using ELISA. Genotyping of the RARRES2 polymorphism was performed using the t-ARMS (allele specific PCR). Allelic and genotypic frequencies were compared among the study groups, and associations with age, BMI and biochemical parameters were evaluated. Results: The distribution of RARRES2 genotypes was significantly varied among the study groups. The GG genotype was more frequently observed in patients with T2DM with MetS [33 (33.7%)] than in patients with T2DM without MetS [12 (12.2%)], with a statistically significant difference (p<0.001). Variations in genotype distribution were also observed across selected age and BMI categories. Level of serum chemerin were elevated in patients with T2DM with MetS in comparison to other study groups. The findings of this study advocate the association of RARRES2 rs1717368 genetic variation with metabolic phenotype of T2DM, particularly in the presence of MetS. Conclusion: This study exhibits a prominent association between RARRES2 rs1717368 gene polymorphism and T2DM with MetS. The higher frequency of the GG genotype in the T2DM with MetS categories suggests that this genetic variant may contribute to susceptibility to metabolic complications. Further studies involving larger and ethnically diverse populations are warranted to confirm these findings.