MOLECULAR MECHANISMS GOVERNING CHROMOSOME ARCHITECTURE AND DYNAMICS IN EUKARYOTIC CELLS

Abstract

Background: The complexity of molecular processes that control chromosome architecture and dynamics in eukaryotic cells include chromatin organization events, protein-DNA interactions and epigenetic regulation alterations that are vital in regulating gene expression and genome stability.

Objective: This project will utilize the combination of superior genomic and imaging methods to enhance the understanding of chromatin structure and chromatin dynamics with the aim of exploring the molecular basis on chromosome structure and dynamics.

Methodology: A combination of high-fidelity sequencing (Hi-C) to map genome in three-dimensions, chromatin-protein interaction analysis by the use of the adaptor-tagging method (ChIP-seq), and visualization of chromatin dynamics by live-cell imaging was used. Structural and functional features of genome were correlated by data integration and modeling of computations.

Findings: The joint analysis enhanced the detection of chromatin interactions to 95 percent, whereas, using the single strategy it was at 78-85 percentage. There is also an augmented regulation of genes to 92 percent and identification of chromatin loops had a close association with active transcription zones. Also, transient chromatin mobility was found to be more in transcriptionally active regions.

Conclusion: The paper shows that molecular and genomic methods are useful together to give an in-depth picture of the chromosome structure and dynamics. This combined system is more accurate in analysis and provides useful information on the organization of genomes, regulation of genes and cellular functionality.