COORDINATION-DRIVEN BIOACTIVITY OF AN OXIME BASED COBALT (II) CHELATE: SYNTHESIS, STRUCTURAL CHARACTERIZATION, AND IN VITRO ANTIOXIDANT AND ANTICOAGULANT EVALUATION

Abstract

A ligand with sulfur and nitrogen atom functionality, 2-prop-2-en-1-yl-N-hydroxyprop-2-ene-1 sulfinimidothioate oxime (L1), was utilized to synthesize a cobalt (II) complex (CoL1) and test its in vitro scavenging ability as well as plasma clotting-time response. Ligand L1 was synthesized through the reaction of S-(prop-2-en-1-yl) prop-2-ene-1-sulfinothioate with hydroxylamine hydrochloride in aqueous alkaline medium. Further reaction between the synthesized ligand and a cobalt (II) salt in methanol, while heating under mildly acidic to nearly neutral pH condition yielded CoL1. The synthesized compound was tested for its DPPH radical inhibition and plasma clotting activity from 100 to 500 µg/mL. Inhibition of DPPH increased from 20.39% at 100 µg/mL to 70.82% at 500 µg/mL, while sample absorbance decreased from 0.0652 to 0.0239 in comparison to the control absorbance of 0.0819. In the plasma assay, clotting time increased from 44 s (ratio 1.38) to 102 s (ratio 3.19) compared to the control of 32 s (ratio 1.00). These responses in terms of concentrations suggest that CoL1 is a candidate compound for further study. These data represent descriptive screening results. Replicate numbers, variances, statistical analysis and interference controls were unavailable. Therefore, no mechanism of action, safety or efficacy could be determined from these observations.