DETECTION AND CHARACTERIZATION OF VIRULENCE AND RESISTANCE DETERMINANTS (SPA, LUKS/F-PV, MECA) IN S. AUREUS FROM MASTITIC COW MILK

Abstract

One of the main reasons that limits therapeutic effectiveness against bovine mastitis, which is probably one of the most important diseases affecting dairy cattle worldwide in economic terms, is antibiotic resistance and Streptococcus aureus represents a large percentage of all clinical and subclinical mastitis cases. The present study was designed to molecular detection and characterization of certain virulence and antimicrobial resistance genes (spa, many lukS/F-PV, mecA) in Staphylococcus aureus isolates from mastitis cow's milk in Diyala Governorate. From which, 100 mastitis milk samples were recorded from affected free-stall loose-housed cows with clinical mastitis. Molecular positivity rate were 30.0%, total 30 positive isolates have been identified from mastitis milk samples as tested they are found to be positive. In conventional PCR analysis-all of the spa among 30 (100 %), mecA among 19 (63.3 %) and lukS/F-PV positve isolates-indicated7(23.3%). The detection of virulence- and methicillin-resistance-genes contributes to the confirmation of circulation in strains isolated from bovine mastitis. At Macrogen Company, selected PCR positive amplicons of similar residues exhibited 99% or greater homology with reference strains in GenBank as indicated by a combination of sequencing and sequence alignment. All of the sequences generated anew were deposited with GenBank accession numbers PQ123456-PQ123469. Our results highlight the necessity of molecular features and epidemiological surveillance using PCR and sequencing to monitor bovine mastitis-associated S. aureus isolates.