BIOLOGICAL EVALUATION OF SILVER NANOPARTICLES USING AGLAOMORPHA QUERCIFOLIA PLANT EXTRACT: ANTIBACTERIAL, ANTIOXIDANT, ANTICANCER, AND ANTI INFLAMMATORY ACTIVITIES

Abstract

The present study investigates the green synthesis of silver nanoparticles (Ag-NPs) using the methanolic extract of Aglaomorpha quercifolia and evaluates their biological activities. The synthesized Ag-NPs exhibited significant antibacterial activity against both Gram-positive and Gram-negative bacteria, with maximum inhibition observed against Corynebacterium glutamicum at 150 µg/mL. The nanoparticles also demonstrated potent anticancer activity against HCT 116 human colorectal cancer cells, with an IC₅₀ value of 57.81 ± 0.19 µg/mL and concentration-dependent cytotoxicity. Antioxidant potential was confirmed through DPPH and FRAP assays, showing enhanced free radical scavenging activity with increasing nanoparticle concentration. Furthermore, the Ag-NPs displayed remarkable anti-inflammatory activity, achieving 80% inhibition at 50 µg/mL. These findings suggest that Aglaomorpha quercifolia-mediated silver nanoparticles possess promising biomedical applications as antimicrobial, antioxidant, anticancer, and anti-inflammatory agents. Further in vivo studies are warranted to validate their therapeutic potential.