THE EFFECT OF CINNAMON EXTRACT ON HORMONAL PARAMETERS IN METHOTREXATE INDUCED INFERTILITY IN FEMALE ALBINO RATS

Abstract

Objectives: To study the effect of cinnamon Extract on serum FSH, LH and AMH level in Methotrexate-induced infertility in albino rat.

Material & Methods: Prospective experimental animal study was done on 27 healthy female adult albino rats of 8-10 weeks, weighing 120-180gm, from the animal house of Baqai Medical University during February 2025 till August 2025. The rats were randomly divided into three groups: A, B and C, each group consisting of 9 animals. The animals from each group were kept in separate cages. Each group further divided into three sub groups, each subgroup contains 3 animals. Group-A (Control group) all subgroups A1, A2 and A3 was given normal saline intraperitoneally daily. Group-B (Methotrexate-induced group) all subgroups B1, B2 and B3 was given Methotrexate 2.5mg/kg intraperitoneally daily. Group C (Methotrexate plus Cinnamon-protected group) all subgroups C1, C2 and C3 was given Methotrexate 2.5mg/kg intraperitoneally daily plus Cinnamon Extract l00mg/kg intraperitoneally daily. Rats will be sacrificed at the end of their respective period of time i.e. 2^nd, 4^th and 6^th weeks, blood was taken from intra cardiac puncture and analyzed through ELISA.

Results: All sub-groups within the methotrexate- induced group exhibited a marked reduction in body weight as compared with control and cinnamon-protected group. FSH and LH revealed statistically significant differences, as indicated by the p-value of <0.01.  FSH and LH is significantly increased with methotrexate-induced group as compared with cinnamon-protected group. The methotrexate-induced group exhibited substantially lower AMH levels across all subgroups, while the cinnamon-protected group demonstrated AMH levels slightly lower than the control group, suggesting that the protective intervention effectively preserved ovarian reserve and reduced the decline observed in the methotrexate-induced group.

Conclusion: Cinnamon Extract could prevent the hormonal imbalance in Methotrexate induced infertility in albino rats.