COMPARISON STUDY BETWEEN MARJORAM (ORIGANUM MAJORANA L.) AND THYME (THYMUS VULGARIS L.) OILS AGAINST GENTAMYCIN-INDUCED NEPHROTOXICITY IN RATS

Abstract

Background: Gentamicin-induced kidney failure is primarily caused by oxidative damage, inflammation, and apoptosis. Therefore, marjoram contains phenolic terpenoids including (thymol and carvacrol), flavonoids, tannins, hydroquinone, phenolic glycosides, moreover, thyme oils contain thymol and carvacrol as their main bioactive components that all considered preventive agents against gentamycin-induced nephrotoxicity in rats. Objective: To compare the effects of Marjoram (Origanum majorana L.) and Thyme (Thymus vulgaris L.) Oils Against gentamycin-induced nephrotoxicity in rats. Materials and Methods: Fifty five male albino rats weighing 200±5g were divided into equal five groups (n=5 rats); one kept as normal control negative (C -ve), while the rest four groups were via intra-peritoneal injected by gentamicin at a dose of 100 mg/kg b. wt., for 7 days to induce nephrotoxicity; one group left as a positive control (C +ve), while the rest three groups fed on 1 mL/kg b. wt., of marjoram, thyme oils and mixture of them each, for 45 consecutive days. At the end of experiment, body weight gain, food intake, feed efficiency ratio and relative organs weights were determined, Moreover, kidney functions (creatinine, urea and uric acid), total protein, albumin and globulin were determined, as soon as histopathological examinations of kidneys had been measured. Data were analyzed using the Statistical Package for Social Sciences (SPSS) ver. 28. Results: Data showed a significant decrease in body weights gain and relative organs weights. Moreover, there was a significant decrease in creatinine, urea, uric acid, total protein, albumin and globulin as soon as noticeable kidneys histopathological improvement. Conclusion: It was proven that marjoram and thyme oils could be used as treatment agents for gentamycin-induced nephrotoxicity in rats indicating more effectiveness for thyme oil.