¬PHYTOCHEMICAL PROFILING AND IN-VIVO ANTIOXIDANT SCREENING OF GREWIA ASIATICA LEAVES EXTRACTS

Abstract

Background: Grewia asiatica is a berry plant, commonly called phalsa of Malvaceae family. The literature suggests that the plant is popular in folk medicines.

Aim: The study aimed to explore the pharmacognostical and radicle scavenging profile of Grewia asiatica leaves.

Methodology: Study uses a multidimensional methodology to understand the Phytochemistry and analyze its medicinal potential. For this design, plant morphology, physiochemical properties, preliminary TLC, SOD (Superoxide dismutase), GSH (glutathione) and LPO (Lipid peroxidation) methods were employed.

Result and Discussion: The study indicates that the plant has cordate leaves with pointed apex. The percentage yield of aqueous and methanolic extract was 33.238gms and 15.335gms, respectively. Preliminary phytochemical study verified carbohydrate, protein, tannins, and glycosides, along with considerable concentration of flavonoid, phenol and alkaloid in both extracts, highly in methanolic extract. TLC of methanolic extract confirms the presence of crucial constituents, prioritizing the molecular heterogeneity. In the CCl₄ treated animals, the significantly induction of oxidative stress was demonstrated in 28 days study. The treatment animals with 100, 200 and 400mg/kg/day G. asiatica methanolic leaves extract  are evidenced to balance free radicles by reduced LPO, increased intracellular GSH and SOD levels. The treatment in the dose of 200 and 400mg/kg was significantly produce similar results as produce by standard drug silymarin.

Conclusion: The study demonstrated the Pharmacognostical and therapeutic standards of Grewia asiatica with laying the foundation for the forthcoming medicinal investigation moreover, patient oriented investigation.