PREPARATION, OPTIMIZATION, IN-VITRO AND IN-VIVO EVALUATION OF FUNCTIONALIZED CARBON NANOTUBES PREDNISOLONE CONJUGATES

Abstract

This research focuses on developing a novel nanocarrier-based drug delivery system for epilepsy using polyethylene glycol (PEG)-functionalized multi-walled carbon nanotubes (MWCNTs) loaded with Prednisolone. The primary goal was to create a PEG–MWCNT–Prednisolone conjugate that enables sustained and controlled drug release, thereby enhancing therapeutic effectiveness and patient compliance. The MWCNTs were functionalized through a non-covalent method to improve their dispersion stability and drug-loading efficiency. Formulation optimization was conducted using a Design of Experiments (DOE) approach based on the Taguchi method to identify key parameters influencing performance. The conjugates were characterized using Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). FT-IR confirmed successful attachment of Prednisolone to the functionalized MWCNTs, while SEM images revealed distinct morphological changes on the nanotube surface after drug loading. Particle size analysis showed values ranging from 85 to 650 nm, and a zeta potential of +24 mV indicated good stability of the formulation. The drug entrapment efficiency exceeded 70%, and the cumulative in vitro drug release reached approximately 89%, suggesting an effective sustained-release profile. The experimental design employed two factors at three levels, arranged in an L27 orthogonal array to ensure comprehensive evaluation of formulation variables. The anti-allergy activity produced by optimized formulation show significant increased intact cells in mast cell stabilizing. Statistical analysis validated the consistency and reliability of the experimental data. Overall, the PEG-functionalized MWCNT–Prednisolone conjugate showed excellent dispersion, stability, and prolonged release characteristics, indicating its strong potential as an advanced drug delivery system for allergy therapy. This study highlights the promising role of functionalized carbon nanotubes in improving the pharmacological performance of conventional anti-allergy drugs.