PHYTOCHEMICAL ANALYSIS OF INDIVIDUAL AND SYNERGISTIC EXTRACT FRACTIONATION OF CURCUMA CASSIA AND BERBERIS LYCEUM USING HPLC, GC-MS, FTIR AND NMR TECHNIQUE

Abstract

Background: In the current study, we performed the phytochemical analysis of the individual and synergistic combination of Curcuma cassia, Berberis lyceum and their fractions to investigate their active constituents.

Objective: The objective of this study was to identify the major bioactive secondary metabolites present in Curcuma cassia and Berberis lyceum.

Methodology: The hydroalcholic extract of Curcuma cassia and Berberis lyceum were prepared by Soxhlet separately and synergically in an equal proportion. Crude synergistic extract was sequentially fractionated in n-hexane, chloroform and ethyl acetate in order of polarity. Qualitative phytochemical screening was done by standard tests, quantitative analysis of bioactive compounds by HPLC, GC-MS, FTIR, and NMR spectroscopy.

Results: We obtained 34.8% yield from synergistic extract and in fractions ethyl acetate fraction shows maximum yield 29.4%. We also identify the constituents present by qualitative phytochemical analysis. Through HPLC we identify many compounds, Cinnamic acid, coumarins; Tannins are the important one which we targeted. GC-MS also detect Cinnamaldehyde and coumarin, FTIR is used for detecting compounds and NMR analysis identify and quantify molecules type and their shifts.

Conclusion: Our study provided scientific support to researchers with strong background of the active compounds present in Curcuma cassia and Berberis lyceum.