EVALUATION OF ANTIOXIDANT POTENTIAL OF PROPRANOLOL HCL & ASCORBIC ACID COMBINATION FORMULATION USING IN VITRO CHEMICAL METHODS

Abstract

Amplified Oxidative stress impart a serious role in the pathophysiology of hypertension, associated cardiovascular complications, interruption in several intracellular signaling, DNA damages and genomic integrity. Various antihypertensive drugs and their metabolites reduce oxidative stress and plays a major in balancing ROS level. Combining antihypertensive agents with antioxidants may offer synergistic therapeutic benefits and increased influence on oxidative response. This study aimed to evaluate the in vitro antioxidant potential of a combination formulation containing Propranolol HCl and Ascorbic acid using established chemical methods. The antioxidant activity was assessed using (DPPH) radical scavenging assay, Superoxide Anion Scavenging Assay, Hydrogen peroxide scavenging activity, ABTS Radical Cation Decolorization Assay, Ferric Reducing Antioxidant Power (FRAP) Assay, Hydroxyl Radical Scavenging Assay. The combination formulation was compared against individual components and standard antioxidants. The combination formulation demonstrated enhanced antioxidant activity compared to individual components. The IC₅₀ values for DPPH scavenging were 1052.93 µg/mL for the combination versus 1269.62 µg/mL for Propranolol HCl alone and 1473.55 µg/mL for Ascorbic acid alone. The Propranolol HCl–Ascorbic acid combination exhibited significant antioxidant potential, suggesting potential therapeutic advantages in managing hypertension-associated oxidative stress and other cellular genomic damages due to oxidative stress. These findings support further investigation in biological models.