MODULATION OF OXIDATIVE STRESS AND CARDIAC BIOMARKERS IN ISO-INDUCED MYOCARDIAL INFARCTION IN RATS: ROLE OF BONE MARROW-DERIVED MESENCHYMAL STEM CELL THERAPY

Abstract

Background: Isoproterenol-induced MI is associated with oxidative stress and disruption of membrane integrity. BM-MSCs have shown promising cardio-protective and reparative effects through immunomodulatory and antioxidant mechanisms. The current study aims to evaluate the role of BM-MSC in modulating oxidative stress and cardiac biomarkers in ISO-induced MI in rats.

Methods: BM-MSCs were isolated from the femur and tibia of rats and cultured. 30 SD male rats were divided into 3 groups. G1 (control), G2 (MI group), and G3 (BM-MSC treated group). A fresh ISO solution was prepared. MI was induced by S.C. administration of 85 mg/kg/day of ISO for 2 consecutive days. After the last dose of ISO, BM-MSC were administered I/V to G3. On the 28^th day, rats were euthanized, and blood and heart tissue samples were obtained. Oxidative stress, antioxidants, and cardiac biomarkers were measured, and RT-PCR was performed to estimate cardiac gene expression. Histopathology of heart tissue was performed.

Results: ISO administration resulted in significantly increased levels of oxidative stress and cardiac-specific biomarkers, as well as increased expression of cardiac-specific genes. BM-MSC treatment was associated with significant declines in oxidative stress and cardiac biomarker levels, as well as decreased expression of cardiac genes. Significantly elevated antioxidant levels were observed with BM-MSC treatment. Histology showed improved myocardial architecture in G3.  

Conclusion: BM-MSC therapy modulates oxidative stress and cardiac biomarkers, enhances antioxidant defense, and thus serves as a promising therapeutic strategy for ISO-induced MI.