ANTINEMATODAL POTENTIAL OF THIOSEMICARBAZIDE (T-15) AND TRIAZOLE (TR-19) CLASSES OF SYNTHETIC COMPOUNDS USING CAENORHABDITIS ELEGANS AS A MODEL ORGANISM

Abstract

Nematodes are the helminthes they can cause parasitic infections in humans. They are responsible for causing substantial mortality and morbidity in humans and animals throughout the tropical and sub-tropical worlds. They are also responsible for losses in livestock and crop damage of more than US$100 billion annually. Several drugs are being used against these nematodes but selected Synthetic compounds, thiosemicarbazides (T-15 ) and Triazoles (TR-19) Antinematodal potential at the cellular level has been introduced DMSO is used for dilutions. In mostly cell biology Caenorhabditis elegans used as model organism. Fluorescence microscopies and Anti-egg hatching of nematode were done.) Thiosemicarbazides (T-15) (EC50 = 0.2 mg/mL) and Triazoles (TR-19) (EC50 = 1.27 mg/mL synthetic compounds showed fine antinematodal activity.  These Synthetic compounds reflect excellent anti-egg hatching activity with fine concentration of 1mg/mL. Thiosemicarbazides (T-15) and Triazoles (TR-19)   DMSO dilutions exhibited excellent apoptosis effects in intestines, uterus (eggs), and muscles, gonads. q PCR is used to identify the gene expression of treated genes. Both genes gst-4 and hsp-6 shows strong gene expression during q-PCR after being treated with synthetic compounds. And Thiosemicarbazides (T-15) and Triazoles (TR-19) for potent broad-spectrum antinematodal medications.