COMPARISON OF AN INDUCTION MODELS FOR THE DEVELOPMENT OF PSYCHOSIS CONDITION AND ITS ASSESSMENT TARGETING D2 RECEPTORS BY PRECLINICAL STUDIES

Abstract

Introduction: Psoriasis is an autoimmune, progressive, and chronic inflammatory condition predominantly caused by various inflammatory and immunological mediators. Phytochemicals, such as plumbagin (PL) and resveratrol (RSV), have anti-inflammatory, antioxidant, and immunomodulatory properties.

Methods: The induction of psoriasis-like conditions in rats was standardized by exposing them to ultraviolet (UV) radiation for 30 minutes  on their dorsal skin surfaces. The  psoriasis area severity index (PASI) scoring  was  utilized  to  confirm  the  psoriasis  induction. Biochemical measurement of the hydroxyproline  (HP) content was performed to  measure collagen rupture, accompanied by histological evaluation of the exposed area. The Swiss Dock web server was used for the in silico docking of mammalian targets  of rapamycin (mTOR), fibro-collagenase, and nuclear factor kappa  B  proteins. Results: A 30-minute UV exposure was standardized and created as a model for inducing psoriasis-like conditions. Administration of PL and RSV alone or in combination restored the increased PASI score within  a  week and hastened recovery.  These medications  also limited the increase in the HP content and altered the morphology. The combination of PL with RSV is more effective than either

medicine alone. In silico docking, the analysis demonstrated that PL and RSV had  a  good binding

relationship with mTOR, NF-κB, and fibro-collagenase.

Conclusion: UV exposure for 30 minutes causes a psoriasis-like state, whereas PL and RSV prevent the production and progression of psoriasis-like conditions via their multi-target modulatory mechanisms. Target-specific investigations require molecular-level research. Keywords Psoriasis, Plumbagin, Resveratrol, UV-Induced Psoriasis, Molecular Docking Preclinical animal models are essential for elucidating the neurobiological mechanisms underlying psychosis, stress-related psychopathology and for evaluating potential therapeutic agents. Among the various experimental paradigms, Chronic Unpredictable Mild Stress (CUMS), Olfactory Bulbectomy (OBX) and Dexamethasone-induced glucocorticoid exposure model are widely employed in rats to behavioural, neuroendocrine, and neurochemical alterations relevant to depressive-like states leading to cause depression induced psychosis. The behavioural parameters such as hyperactivity, stereotypy, etc., biochemical estimation such as dopamine, lipid peroxidation and histopathological studies were studied.It was concluded that among all the three models of induction- olfactory bulbectomy induces psychosis model had shown the good result.