Use of an electrophoresis box made with inexpensive materials to verify DNA from Escherichia coli

E.B. Santana, R.S. Santos
Published: March 11, 2024
Genet. Mol. Res. 23(1): GMR19156
DOI: https://doi.org/10.4238/gmr19156

Cite this Article:
E.B. Santana, R.S. Santos (2024). Use of an electrophoresis box made with inexpensive materials to verify DNA from Escherichia coli. Genet. Mol. Res. 23(1): GMR19156. https://doi.org/10.4238/gmr19156

About the Authors
E.B. Santana, R.S. Santos
Corresponding Author
Email: ebrazilino@uneb.br

ABSTRACT

Experimental classes may aid to develop scientific concepts and allow students to understand how to objectively approach their reality and develop solutions for complex problems, as environmental pollution and support for high schools and universities laboratories. In this work an electrophoresis box was made by students from and acrylic chocolate box, to verify DNA extracted and amplified from E. coli. Disposable cell phone power chargers of 12 V capacity were used as energy source. DNA fragments migrated during the electrophoresis process, demonstrating this artisanal box may be used for molecular biology experiments in schools and universities. Beside the possibility of using inexpensive laboratory material a reduction of environmental impacts, due to disposed materials such cell phone chargers and plastic containers, is also an advantage.

Key words: Electrophoresis, Environmental Impacts, Experimental Classes, Low Cost Materials.

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