E.V. Adamowski, K.R. Boldrini, M.S. Pagliarini, C.B. do Valle
Published September 30, 2007
Genet. Mol. Res. 6 (3): 616-621 (2007)

Corresponding author
M.S. Pagliarini
E-mail: mspagliarini@uem.br

ABSTRACT

Microsporogenesis was evaluated in the Brachiariahu-midicola collection of the Embrapa Beef Cattle Center, represented by 60 accessions. One accession (H121) presented an abnormal pattern of cytokinesis that had never been reported in this genus. Among 900 meiocytes analyzed in the first division, 10.7% underwent precocious and multiple cytokinesis in metaphase I, fractionating the genome and the cytoplasm into two or more parts. The expected cytokinesis after telophase I did not occur. The abnormal meiocytes from the first division entered the second division but the second cytokinesis after telophase II was also abnormal. Among the 857 meiocytes analyzed in the second division, 10.9% presented abnormal, incomplete or total absence of cytokinesis. Dyads and binucleatedmicrospores were recorded among the meiotic products. The use of this accession in the Embrapa breeding program is compromised.

Key words: Brachiaria humidicola, Breeding program, Cytokinesis, Forage grass, Microsporogenesis