G. Thedei Junior, A. Rossi
Published August 7, 2006
Genet. Mol. Res. 5 (3): 483-486 (2006)

About the authors
G. Thedei Junior, A. Rossi

Corresponding author
A. Rossi
E-mail: anrossi@usp.br

ABSTRACT

The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three nonspecific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to either Mg2+ or EDTA.Another alkaline phosphatase activity, whose location in the hyphal cell is regulated by phosphate, is stimulated by Mg2+, inhibited by EDTA, and somehow dependent on the expression of the pho-2+ -encoded Pi-repressible alkaline phosphatase.

Key words: Alkaline phosphatase, Gene regulation, Neurospora crassa, Phosphate sensing