P.V. Tiago, M.P. Carneiro-Leão, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima
Published: August 5, 2011
Genet. Mol. Res. 10 (3): 1565-1575
DOI: http://dx.doi.org/10.4238/vol10-3gmr1069

Cite this Article:
P.V. Tiago, M.P. Carneiro-Leão, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima (2011). Genetic characterization of Metarhizium anisopliae var. anisopliae isolates from Mahanarva fimbriolata, a sugarcane pest in Brazil. Genet. Mol. Res. 10(3): 1565-1575. http://dx.doi.org/10.4238/vol10-3gmr1069

About the Authors:
P.V. Tiago, M.P. Carneiro-Leão, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima
Corresponding author: P.V. Tiago
E-mail: patiago@hotmail.com

ABSTRACT
Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará da Serra, Southwest Mato Grosso State, Brazil. Internal transcribed spacer-RFLP did not differentiate the isolates of M. anisopliae var. anisopliae, while ISSR and intron primers identified three distinct groups. Variability among these groups was 96% for (GTG)5 and 100% for the other primers. We found considerable genetic variability, even among isolates from the same geographical origin and host.

Key words: Metarhizium anisopliae, Mahanarva fimbriolata, ITS-RFLP, ISSR, Intron.