X.J. Bu, L. Liu, L. Wang, L.W. Nie
Published: May 31, 2011
Genet. Mol. Res. 10(2): 1006-1010
DOI: https://doi.org/10.4238/vol10-2gmr1119

Cite this Article:
X.J. Bu, L. Liu, L. Wang, L.W. Nie (2011). Isolation and characterization of 21 novel polymorphic microsatellite loci in the Chinese soft-shelled turtle Pelodiscus sinensis. Genet. Mol. Res. 10(2): 1006-1010. https://doi.org/10.4238/vol10-2gmr1119

About the Authors
X.J. Bu, L. Liu, L. Wang, L.W. Nie
Corresponding Author: L.W. Nie
Email: lwnie@mail.ahnu.edu.cn

ABSTRACT

Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and detected high levels of polymorphism for all 21 loci, with the number of alleles/locus ranging from 5 to 23 (mean 15.67). The expected and observed heterozygosities ranged from 0.421 to 0.946 and from 0.324 to 0.941, respectively. Hence, these microsatellites could facilitate studies on genetic diversity and population structure and marker-assisted breeding of this vulnerable species.

Key words: Pelodiscus sinensis, Microsatellites, Genetic diversity, Conservation.