C. Clezar, L.I. Weber, G.S. Malaquias, P.O. Silva
Published: June 24, 2008
Genet. Mol. Res. 7 (2) : 567-572
DOI: https://doi.org/10.4238/vol7-2gmr459

Cite this Article:
C. Clezar, L.I. Weber, G.S. Malaquias, P.O. Silva (2008). Evidence for the main foot protein gene in Perna perna (Mollusca, Mytilidae). Genet. Mol. Res. 7(2): 567-572. https://doi.org/10.4238/vol7-2gmr459

About the Authors
C. Clezar, L.I. Weber, G.S. Malaquias, P.O. Silva

Corresponding author
L.I. Weber
E-mail: liweber@nupem.ufrj.br

ABSTRACT

Here, we investigated the gene that encodes the main protein component of the byssus in Perna perna (Ppfp) using a consensus fluorescent probe for the Mytilus group and by polymerase chain reaction amplification using specific and random primers. A 524-bp fragment resulting from polymerase chain reaction amplification was found to be homologous to the fp-1 gene of Mytilus coruscus. This fragment was identified as the 3’ end of the Ppfp-1 gene, which included the heptapeptide coding sequence for Lys-Pro-Ser-Tyr-Pro-Pro-Ter (probably the incomplete last tandem repeat unit of the giant exon), the stop codon and the polyadenylation signal.

Key words: Perna perna, byssus foot proteins, fp-1 gene, polyphenolic protein.