I.G. Hamoy, E.J.M. Santos, S.E.B. Santos
Published: January 22, 2008
Genet. Mol. Res. 7 (1) : 29-32
DOI: https://doi.org/10.4238/vol7-1gmr394
Cite this Article:
I.G. Hamoy, E.J.M. Santos, S.E.B. Santos (2008). Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites. Genet. Mol. Res. 7(1): 29-32. https://doi.org/10.4238/vol7-1gmr394
About the Authors
I.G. Hamoy, E.J.M. Santos, S.E.B. Santos
Corresponding author
I.G. Hamoy
E-mail: ighamoy@yahoo.com.br
ABSTRACT
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.
Key words: Microsatellites, Multiplex polymerase chain reaction, Arapaima gigas, pirarucu, conservation genetics,Lower Amazon.