Faria Vieira, Ariadna, Pantalião, Gabriel Feresin, Martins Abreu, Fernanda, Silveira, Ricardo Dias, Basso Garcia, Ana Letycia, Gomide Neves, Leandro, Pereira Vianello, Rosana, Pereira Castro, Adriano, Brondani, Claudio
Published: February 27, 2018
Genet. Mol. Res. 17(1): gmr16039886
DOI: https://doi.org/10.4238/gmr10.4238/gmr16039886
Cite this Article:
A.Faria Vieira, G.Feresin Pantalião, F.Martins Abreu, R.Dias Silveira, A.Letycia Ba Garcia, L.Gomide Neves, R.Pereira Vianello, A.Pereira Castro, C. Brondani (2018). Marker-Assisted elimination of drought-susceptible accessions in upland rice breeding. Genet. Mol. Res. 17(1): gmr16039886. https://doi.org/10.4238/gmr10.4238/gmr16039886
About the Authors
Faria Vieira, Ariadna, Pantalião, Gabriel Feresin, Martins Abreu, Fernanda, Silveira, Ricardo Dias, Basso Garcia, Ana Letycia, Gomide Neves, Leandro, Pereira Vianello, Rosana, Pereira Castro, Adriano, Brondani, Claudio
Corresponding Author
Claudio Brondani
Email: claudio.brondani@embrapa.br
ABSTRACT
Breeding for water-deficit tolerance is fundamental to guarantee the sustainability of upland rice production, mainly due to the possibility of an increased frequency of drought episodes due to climate change. This work aimed to identify single nucleotide polymorphism (SNP) markers, derived from RNA sequencing (RNA-Seq), genome-wide association study (GWAS) and candidate genes from Arabidopsis, with potential for use in marker-assisted selection (MAS) for drought tolerance. RNA-Seq and GWAS were efficient in identifying useful SNP markers from the data obtained from three years of field experiments for 175 upland rice accessions, which were sequenced using 32 genes by Capture-Seq. Three genes were equally able to generate SNP markers that discriminated 95% of the 20 most drought susceptible accessions in the joint analysis of the experiments. The elimination of the genotypes with the unfavourable SNP allele reduced the initial number of accessions to one third, and transferring this result in a breeding routine, would enable to conduct smaller experiments per target location, increasing the precision and reducing the cost of the drought phenotyping.
Key words: Capture-Seq; drought tolerance, Genotyping by Sequencing, Oryza sativa L, RNA-seq, RNA-Seq, SNPs.