M.N. Cardoso, A.L.S. Nascimento, L.A.R. de Oliveira, D.A. de Assunção, C.A. Machado, A.C.A. de Oliveira, A.S. de Jesus, A.S. Lédo, R.S. Archiminio, A.R.C. Rabbani, A.V.C. Silva
Published: February 26, 2019
Genet. Mol. Res. 18(1): GMR18119
DOI: https://doi.org/10.4238/gmr18119
Cite this Article:
M.N. Cardoso, A.L.S. Nascimento, L.A.R. de Oliveira, D.A. de Assunção, C.A. Machado, A.C.A. de Oliveira, A.S. de Jesus, A.S. Lédo, R.S. Archiminio, A.R.C. Rabbani, A.V.C. Silva (2019). Genetic diversity in native Genipa americana (Rubiaceae) populations in Sergipe, Brazil. Genet. Mol. Res. 18(1): GMR18119. https://doi.org/10.4238/gmr18119
About the Authors
M.N. Cardoso, A.L.S. Nascimento, L.A.R. de Oliveira, D.A. de Assunção, C.A. Machado, A.C.A. de Oliveira, A.S. de Jesus, A.S. Lédo, R.S. Archiminio, A.R.C. Rabbani, A.V.C. Silva
Corresponding Author
M.N. Cardoso
Email: milenacardoso.bio@gmail.com
ABSTRACT
Genipap (Genipa americana – Rubiaceae) is native to tropical America, occurring in Brazil in practically all biomes, except in the Pampas. It has socioeconomic importance, mainly due to the use of fruits in the manufacture of sweets and juices besides the medicinal properties of its leaves. We evaluated the genetic diversity of 73 individuals from 15 natural populations in the state of Sergipe using ISSR markers. The choice of areas was made randomly and genetic material was extracted from young leaves. PCR analysis using nine markers generated 113 fragments, which were used to estimate genetic diversity. The Shannon index was 0.36 and the similarity of individuals varied between 0.11 and 0.81. The individuals Salgado 3 (SAL3) and Salgado 4 (SAL4) were the most similar (0.81) and seven pairs had low similarity (0.11). The genetic distance the UPGMA analysis, which divided individuals into two distinct groups. The level of genetic variability found allows differentiation between genotypes that can be used to enrich the Genipap Active GermPlasm Bank in Sergipe, and the information generated will be useful for the conservation of these genetic resources and in future breeding programs for this species.
Key words: Genipap, ISSR, Plant genetic resources, Populations.