PFG Ferreira, RA Silva , IG Hamoy , LC Marques , CS Silva , JRF Marques , WLA Pereira and E Silva Filho
Published September 16, 2024
Genet. Mol. Res. 23 (3): gmr2377
DOI http://dx.doi.org/10.4238/gmr2377
About the Authors
PFG Ferreira, RA Silva , IG Hamoy , LC Marques , CS Silva , JRF Marques , WLA Pereira and E Silva Filho
Corresponding author:
Palmira Francisca Gonçalves Ferreira
E-mail: palmirafgf@gmail.com
ABSTRACT
Kinosternon scorpioides is a freshwater turtle in need of conservation due to its vulnerability and population decline caused by predatory extraction from the natural environment and habitat destruction and degradation. Consequently, previous studies have focused on indicating the species’ productive potential through productive features associations that aid the captive breeding system, but no one has explored candidate genes for these traits through molecular markers. Therefore, the C-mos gene was evaluated by the polymerase chain reaction technique (PCR) to look for polymorphisms useful in genetic analyses of a population raised in the Embrapa Eastern Amazon captivity, given its role in the maturation and regulation of oocyte transition and skeletal muscle development. The aim was to look for these polymorphisms, characterize them, and link them with body weight (BW) and the average number of eggs (NE) traits. Thus, the DNA of 75 animals was extracted, sequenced, and the allelic and genotype frequency, observed and expected heterozygosity, inbreeding coefficients (FIS), and Hardy-Weinberg probability measures were assessed with GENEPOP software. The analysis of variance (ANOVA) one-way test at a 5% level of significance was used to check if BW and NE traits differ between different genotypes. The results revealed a non-synonymous polymorphism resulting in three genotypes: CC (49%), CT (42%), and TT (9%). However, these genotypes were not statistically associated with the body weight or number of eggs of the animals. Although this polymorphism is suitable to be used as a molecular marker in population genetic analysis involving species raised in captivity.
Key words: Capitivity; Captive breeding; Gene; K. Scorpioides; Polymorphism.