R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He
Published May 18, 2010
Genet. Mol. Res. 9 (2): 931-934 (2010)
DOI 10.4238/vol9-2gmr806

About the authors
R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He

Corresponding author
G.Y. He
E-mail: hegy@hust.edu.cn

ABSTRACT

Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three loci deviated from Hardy-Weinberg equilibrium, and linkage disequilibrium between five pairs of loci was significant. These polymorphic microsatellite loci can be used for genetic diversity analysis and molecular-assisted breeding of M. miiuy.

Key words: Miichthys miiuy; Microsatellite; Molecular marker