Z.S. Ozkan, D. Deveci, E. Onalan Etem and H. Yuce
Published November 30, 2010
Genet. Mol. Res. 9 (4): 2311-2316 (2010)
DOI 10.4238/vol9-4gmr922

About the Authors
Z.S. Ozkan, D. Deveci, E. Onalan Etem and H. Yuce

Corresponding author: 
Z.S. Ozkan
E-mail: zehrasema@yahoo.com

ABSTRACT

We investigated the effect of bone morphogenetic protein 2 and 4 (BMP-2 and -4) gene polymorphisms on bone density in postmenopausal Turkish women with osteoporosis. The frequency of single-nucleotide polymorphisms (SNPs) of BMP-2 and -4 genes was analyzed in 101 osteoporotic-postmenopausal women and 52 postmenopausal women with positive bone mineral density scores. We evaluated the frequency of the thymine→cytosine nucleotide variation at position 538 for BMP-4 and the transposition of adenine→thymine at codon 190 for BMP-2, with PCR. The proportions of genotypes observed for the BMP-2 SNP in the osteoporotic group were AA (47.5%), AT (39.6%), TT (12.9%), and in the non-osteoporotic group they were AA (48.1%), AT (40.4%), TT (11.5%). The corresponding frequencies for the BMP-4 SNP in the osteoporotic group were TT (30.7%), TC (45.5%), CC (23.8%), and in the non-osteoporotic group they were TT (26.9%), TC (40.4%), CC (32.7%). There were no significant differences in the frequencies of these genotypes between the patient and control groups. We conclude that genetic variations in BMP-2 and -4 do not substantially contribute to lumbar spine bone mineral density in postmenopausal Turkish women.

Key words: Bone mineral density; Single-nucleotide polymorphism; Bone morphogenetic protein 2 and 4; Genetic association; Osteoporosis