GPN de Souza, PC Novais, MVB Celani, JP da Silva, JR Lellis, JA Thomazini, VPS Fazan, MFGS Tazima, DPC Tirapelli, MLA Cirino and LF Tirapelli*
Published November 25, 2024
Genet. Mol. Res. 23 (4): gmr2368
DOI http://dx.doi.org/10.4238/gmr2368
About the Authors
GPN de Souza, PC Novais, MVB Celani, JP da Silva, JR Lellis, JA Thomazini, VPS Fazan, MFGS Tazima, DPC Tirapelli, MLA Cirino and LF Tirapelli*
Corresponding author:
L.F. Tirapelli
E-mail: lab.biomol.cirurgia@fmrp.usp.br
ABSTRACT
Cerebrovascular accident (CVA) is a major public health problem and is considered one of the leading causes of death and disability worldwide, which, if left untreated, can result in neuronal damage. In addition, apoptotic processes occur far from the ischemic focus, such as in the cerebellum. Due to the disability caused by the stroke in the individual, rehabilitation is necessary to avoid further damage to the patient’s health. Therefore, the use of physical exercise can be an alternative to this problem, involving passive or active exercises, progressive resistance, balance and postural exercises. As physical exercise has a systemic action, it can influence the expression of biomarkers, such as microRNAs, which are small non-coding RNA molecules that act in transcription and post transcription to regulate gene expression. To evaluate the expression profile of microRNA-34c and BCL-2 gene related to apoptosis in cerebellar tissue after experimental induction of temporary focal cerebral ischemia, associated or not with a model of pre-ischemic physical exercise. A total of twenty-two animals were used, subdivided into 4 experimental groups: control group, physical exercise group, ischemic group, ischemic group physical exercise, were submitted to a treadmill training protocol for four weeks, and the training lasted 30 min a day at a speed of 18m/min. Cerebellar tissue samples were collected for the analysis of apoptosis from the expression of microRNA-34c and the BCL-2 gene, using the real-time PCR methodology. We observed a significant difference in the expression of the BCL-2 gene in the control group in relation to the groups: physical exercise, ischemic and physical exercise + ischemic. We did not observe a significant difference in the expression of microRNA-34c.
Key words: Stroke; microRNAs; Apoptosis; Physical Exercise.