Ying Qian, Jingchan Yao, Lan Wang, Yiping Lin, Hui Zhang and Mingxing Ding*
Published June 28, 2024
Genet. Mol. Res. 23 (2): gmr2313
DOI http://dx.doi.org/10.4238/gmr2313

About the Authors
Ying Qian, Jingchan Yao, Lan Wan, Yiping Lin, Hui Zhang and Mingxing Ding*

Corresponding author: 
Mingxing Ding
E-mail: 20050699@jhc.edu.cn

ABSTRACT

Bronchopulmonary dysplasia (BPD) is associated with high mortality rate due to lung injury. Recent studies indicate that cell therapy is a promising treatment option for BPD. This study aimed to examine whether lung injuries caused by hyperoxia can be mitigated by intratracheal mesenchymal stem cells (MSCs) transplantation.Newborn Sprague Dawley rats were housed in room air or hyperoxic chambers. Human umbilical cord -derived mesenchymal stem cells (hUC-MSCs) in saline were intratracheally transplanted into the rats through a 30-gauge needle syringe. Lung and brain tissues were harvested for experimental analysis. hUC-MSCs entered the lung tissue but not the brain. Larger congestive alveoli in the lung tissue caused by hyperoxia could be attenuated by intratracheal transplantation of hUC-MSCs. Intratracheal transplantation mitigated hyperoxia-induced inflammatory reactions. hUC-MSC transplantation inhibited apoptosis activation and terminal deoxynucleotidyl transferase dUTP nick end labeling positive cells in the lungs.Taken together, this study shows that intratracheal transplantation of hUC-MSCs partially mitigates hyperoxiainduced lung injury.

Key words: Bronchopulmonary dysplasia; Umbilical cord -derived mesenchymal stem cells; Lung injuries; brain injuries