X.H. Dong, M.Y. Li, X.N. Tang, X.L. Zhang and L. Miao
Published: July 5, 2011
Genet. Mol. Res. 10 (3): 1314-1319
DOI: 10.4238/vol10-3gmr1105

Cite this Article:
X.H. Dong, M.Y. Li, X.N. Tang, X.L. Zhang and L. Miao (2011). Characterization of highly polymorphic microsatellite markers in Inimicus japonicus. Genet. Mol. Res. 10(3): 1314-1319. 10.4238/vol10-3gmr1105

About the Authors:
X.H. Dong, M.Y. Li, X.N. Tang, X.L. Zhang and L. Miao
Corresponding author: M.Y. Li
E-mail: limingyun@nbip.net

ABSTRACT
Inimicus japonicus, the devil stinger, has an extensive distribution along the coast of China, Japan and the Korean Peninsula. Nineteen highly polymorphic microsatellite markers were isolated and characterized in I. japonicus. Twenty-eight individuals from a wild population were tested for polymorphism using this set of polymorphic microsatellite markers. The number of alleles per locus ranged from 4 to 14. The ranges of observed and expected heterozygosity were 0.500-0.892 and 0.521-0.910, respectively. Significant deviations from Hardy-Weinberg equilibrium were detected at two loci. To the best of our knowledge, these were the first microsatellite loci characterized from the Synanceiidae; they can be used for estimating genetic diversity, population structure studies, parentage analysis, genetic linkage map construction, germplasm classification and identification, gene identification, quantitative trait loci mapping, and marker-assisted selection in breeding of I. japonicus and other species of this family.

Key words: Inimicus japonicus, Devil stinger, Microsatellite DNA, Genetic diversity.