D. Li, H. Cheng, L. Chen, B. Wu
Published: May 31, 2021
Genet. Mol. Res. 20(2): GMR18751
DOI: https://doi.org/10.4238/gmr18751
Cite this Article:
D. Li, H. Cheng, L. Chen, B. Wu (2021). Differential expression of microRNAs in a hyperoxia-induced rat bronchopulmonary dysplasia model revealed by deep sequencing. Genet. Mol. Res. 20(2): GMR18751. https://doi.org/10.4238/gmr18751
About the Authors
D. Li, H. Cheng, L. Chen, B. Wu
Corresponding Authors: H. Cheng; B. Wu
Emails: chenghanrongsz@163.com; wubenqing783@126.com
ABSTRACT
We examined the biological roles of microRNAs (miRNAs) in the pathogenesis of bronchopulmonary dysplasia (BPD). Neonatal rats were randomly assigned to hyperoxia (85% O2) and normoxia (21% O2) groups, and each group had eight neonatal. Twenty differentially expressed miRNAs were identified by deep sequencing, of which 10 were up-regulated and 10 were down-regulated in the hyperoxia group. A total of 5,794 molecular related to gene ontology functions were enriched, including cell location and biological processes. rno-miR-29b-3p were up-regulated, and rno-miR-322-5p and rno-miR-335 were down-regulated in the hyperoxia sample based on quantitative real-time PCR. In conclusion, BPD appears to be caused by activation of extracellular matrix -receptor interaction, cytokine-cytokine receptor interaction, RNA transport, cell cycle, and cell adhesion molecule pathways. These miRNAs may play a role in the occurrence and development of BPD. Our study provides new insight into the biological processes of BPD.
Key words: Bronchopulmonary dysplasia, Chronic lung disease, Deep sequencing, Inflammatory, miRNA.