Yi-Mei Zang, Ya Gao, Ying Liu, Chun-Sheng Liu
Published: April 15, 2018
Genet. Mol. Res. 17(2): gmr16039905
DOI: https://doi.org/10.4238/gmr16039905

Cite this Article:
Y.M. Zang, Y. Gao, Y. Liu, C.S. Liu (2018). Identification of Clematidis radix et Rhizoma and its adulterants by core haplotype based on the ITS sequences. Genet. Mol. Res. 17(2): gmr16039905. https://doi.org/10.4238/gmr16039905

About the Authors
Yi-Mei Zang, Ya Gao, Ying Liu, Chun-Sheng Liu

Corresponding Author
Chun Sheng Liu
Email: max_liucs@263.net

ABSTRACT

To develop a method to identify Clematidis radix et Rhizoma using sequence similarity and sequence-specific genetic polymorphisms based on the ITS sequences. DNA was extracted from leaves of Clematis mandshurica Rupr and C. hexapetala using a DNA extraction kit. ITS sequences were amplified by PCR, and analyzed in Contig Express, DNAman, and MEGA 5.0. The core haplotype was determined, and similarities between the core and other haplotypes were calculated. In total, 138 ITS sequences of C. mandshurica were obtained with a length of 611 bp. The similarity threshold between C. mandshurica and counterfeit species was 99%. Using specific mutation sites, we could identify C. chinensis, C. hexapetala, and C. mandshurica rapidly and accurately. A new DNA-based method has been established to rapidly and accurately identify Clematidis radix et Rhizoma.

Key words: Clematidis radix et Rhizoma, Core haplotype, Identification threshold, Mutation sites.