K.N. Yogendra, P.H. Ramanjini Gowda
Published: February 27, 2013
Genet. Mol. Res. 12 (1) : 506-518
DOI: https://doi.org/10.4238/2013.January.9.4
Cite this Article:
K.N. Yogendra, P.H.Ramanjini Gowda (2013). Phenotypic and molecular characterization of a tomato (Solanum lycopersicum L.) F2 population segregation for improving shelf life. Genet. Mol. Res. 12(1): 506-518. https://doi.org/10.4238/2013.January.9.4
About the Authors
K.N. Yogendra, P.H. Ramanjini Gowda
Corresponding Author
K.N. Yogendra
Email: yogendrakn@gmail.com
ABSTRACT
Breeding for better quality fruits is a major focus for tomatoes, which are continuously subjected to post-harvest losses. Several methods have been used to improve the fruit shelf life of tomatoes, including the use of ripening gene mutants of Solanum lycopersicum. We developed extended shelf-life tomato hybrids with better quality fruits using ripening mutants. Nine tomato crosses were developed using 3 fruit ripening gene mutants of S. lycopersicum [alcobaca (alc), non-ripening, and ripening inhibitor] and 3 agronomically superior Indian cultivars (‘Sankranti’, ‘Vaibhav’, and ‘Pusaruby’) with short shelf life. The hybrid progenies developed from alc x ‘Vaibhav’ had the highest extended shelf life (up to 40 days) compared with that of other varieties and hybrids. Further, the F2 progenies of alc x ‘Vaibhav’ were evaluated for fruit quality traits and yield parameters. A wide range of genetic variability was observed in shelf life (5-106 days) and fruit firmness (0.55-10.65 lbs/cm2). The potential polymorphic simple sequence repeat markers underlying shelf life traits were identified in an F2 mapping population. The marker association with fruit quality traits and yield was confirmed with single-marker analysis and composite interval mapping. The genetic parameters analyzed in the parents and F1 and F2 populations indicated that the cross between the cultivar ‘Vaibhav’ and ripening gene mutant alc yielded fruit with long shelf life and good quality.
Breeding for better quality fruits is a major focus for tomatoes, which are continuously subjected to post-harvest losses. Several methods have been used to improve the fruit shelf life of tomatoes, including the use of ripening gene mutants of Solanum lycopersicum. We developed extended shelf-life tomato hybrids with better quality fruits using ripening mutants. Nine tomato crosses were developed using 3 fruit ripening gene mutants of S. lycopersicum [alcobaca (alc), non-ripening, and ripening inhibitor] and 3 agronomically superior Indian cultivars (‘Sankranti’, ‘Vaibhav’, and ‘Pusaruby’) with short shelf life. The hybrid progenies developed from alc x ‘Vaibhav’ had the highest extended shelf life (up to 40 days) compared with that of other varieties and hybrids. Further, the F2 progenies of alc x ‘Vaibhav’ were evaluated for fruit quality traits and yield parameters. A wide range of genetic variability was observed in shelf life (5-106 days) and fruit firmness (0.55-10.65 lbs/cm2). The potential polymorphic simple sequence repeat markers underlying shelf life traits were identified in an F2 mapping population. The marker association with fruit quality traits and yield was confirmed with single-marker analysis and composite interval mapping. The genetic parameters analyzed in the parents and F1 and F2 populations indicated that the cross between the cultivar ‘Vaibhav’ and ripening gene mutant alc yielded fruit with long shelf life and good quality.
Key words: Composite interval mapping, Fruit firmness, Shelf life, Simple sequence repeats, Single marker analysis, Simple sequence repeats.