E. Maltas, H.C. Vural
Published: February 08, 2013
Genet. Mol. Res. 12 (1) : 408-415
DOI: https://doi.org/10.4238/2013.February.8.5
Cite this Article:
E. Maltas, H.C. Vural (2013). Comparison of winter and summer canola (Brassica napus) genotypes in Turkey. Genet. Mol. Res. 12(1): 408-415. https://doi.org/10.4238/2013.February.8.5
About the Authors
E. Maltas, H.C. Vural
Corresponding Author
E. Maltas
Email: esramaltas@gmail.com
ABSTRACT
We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD markers; 156 reliable bands were found for both genotypes, of which 24% were polymorphic. Fifteen primers gave at least one consistent polymorphic band. The dendogram developed by pooling data of RAPD analysis of summer and winter genotypes had similar patterns. This technique allowed us to examine the relationship between canola genotypes.
We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD markers; 156 reliable bands were found for both genotypes, of which 24% were polymorphic. Fifteen primers gave at least one consistent polymorphic band. The dendogram developed by pooling data of RAPD analysis of summer and winter genotypes had similar patterns. This technique allowed us to examine the relationship between canola genotypes.
Key words: Brassica napus L., Canola, Biodiversity, Genomic DNA, RAPD-PCR.