G.P. Li, C.Q. Zhang and F.L. Cao
Published January 24, 2013
Genet. Mol. Res. 12 (1): 175-182 (2013)
DOI http://dx.doi.org/10.4238/2013.January.24.10
About the authors
G.P. Li, C.Q. Zhang and F.L. Cao
Corresponding author
F.L. Cao
E-mail: samcaoster@gmail.com
ABSTRACT
Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagram that consisted of polymorphic bands produced from different RAPD primers was reported. To check the reliability and efficiency of the cultivar identification diagram, 5 randomly chosen cultivars were further tested, and the workability of the diagram was verified. This new approach will be very helpful for Ginkgo cultivar discrimination and protection, and will also be beneficial for the nursery industry for early identification of Ginkgo seedlings.
Key words: Ginkgo biloba; Cultivar identification; RAPD markers