C. Evrensel, S. Yilmaz, A. Temel, N. Gozukirmizi
Published: May 31, 2011
Genet. Mol. Res. 10(2): 980-987
DOI: https://doi.org/10.4238/vol10-2gmr965
Cite this Article:
C. Evrensel, S. Yilmaz, A. Temel, N. Gozukirmizi (2011). Variations in BARE-1 insertion patterns in barley callus cultures. Genet. Mol. Res. 10(2): 980-987. https://doi.org/10.4238/vol10-2gmr965
About the Authors
C. Evrensel, S. Yilmaz, A. Temel, N. Gozukirmizi
Corresponding Author: N. Gozukirmizi
Email: nermin@istanbul.edu.tr
ABSTRACT
The stability of aging barley calli was investigated with the barley retroelement 1 (BARE–1) retrotransposon specific inter-retrotransposon amplified polymorphism (IRAP) technique. Mature embryos of barley (Hordeum vulgare cv. Zafer-160) were cultured on callus induction MS medium supplemented with 3 mg/L 2,4-D and maintained on the same medium for 60 days. Ten IRAP primers were used in 25 different combinations. The similarity index between 30-day-old and 45-day-old calli was 84%; however, the similarity index between mature embryos and 45-day-old calli was 75%. These culture conditions caused BARE–1 retrotransposon alterations to appear as different band profiles. This is the first report of the use of the IRAP technique in barley in an investigation of callus development.
Key words: BARE-1, Hordeum vulgare L., Tissue culture, Inter-retrotransposon amplified polymorphism.