R. Vasudevan, M.N. Norhasniza, I. Patimah
Published: May 24, 2011
Genet. Mol. Res. 10(2): 943-947
DOI: https://doi.org/10.4238/vol10-2gmr1066

Cite this Article:
R. Vasudevan, M.N. Norhasniza, I. Patimah (2011). Association of variable number of tandem repeats polymorphism in the IL-4 gene with end-stage renal disease in Malaysian patients. Genet. Mol. Res. 10(2): 943-947. https://doi.org/10.4238/vol10-2gmr1066

About the Authors
R. Vasudevan, M.N. Norhasniza, I. Patimah
Corresponding Author: I. Patimah
Email: patimah@putra.upm.edu.my

ABSTRACT

Variable number of tandem repeats (VNTR) polymorphism in the interleukin 4 (IL-4) gene has been associated with end-stage renal disease (ESRD) subjects in many different populations, although with conflicting results. We determined the 70 bp of VNTR polymorphism at intron 3 of the IL-4 gene in Malaysian ESRD subjects. Buccal cells were collected from 160 case and 160 control subjects; genomic DNA was amplified using PCR, followed by agarose gel electrophoresis. There were significant differences in genotypes and alleles of the IL-4 gene. We conclude that VNTR polymorphism of the IL-4 gene is a risk factor for the development of ESRD among Malaysians.

Key words: Genetic polymorphism, VNTR, ESRD, Interleukin.