R.C.P.C. Silva, I.R. Costa, B.M. Bordin, C.T.X. Silva, S.R. Souza, C.L.R. Júnior, A.B. Frare, K.K.V.O. Moura
Published: March 22, 2011
Genet. Mol. Res. 10(1): 465-470
DOI: https://doi.org/10.4238/vol10-1gmr940

Cite this Article:
R.C.P.C. Silva, I.R. Costa, B.M. Bordin, C.T.X. Silva, S.R. Souza, C.L.R. Júnior, A.B. Frare, K.K.V.O. Moura (2011). RsaI polymorphism of the ERβ gene in women with endometriosis. Genet. Mol. Res. 10(1): 465-470. https://doi.org/10.4238/vol10-1gmr940

About the Authors
R.C.P.C. Silva, I.R. Costa, B.M. Bordin, C.T.X. Silva, S.R. Souza, C.L.R. Júnior, A.B. Frare, K.K.V.O. Moura
Corresponding Author: R.C.P.C. Silva
Email: ritagenetica@yahoo.com.br

ABSTRACT

We examined the frequency of RsaI polymorphism of the ERβgene in 54 patients diagnosed with endometriosis and 46 controls. Peripheral blood was collected from women undergoing laparoscopy with a confirmed diagnosis of endometriosis. Polymor­phisms of the ERβgene and p53 were assessed by PCR and analyzed on 2% agarose gel stained with ethidium bromide. The AG poly­morphism genotype frequency in patients with endometriosis was 59.3%, with 40.7% GG. In the control group, the frequency of AG was 6.5%, with 93.5% GG. The frequency of heterozygous AG was nine times higher in patients with endometriosis than in the control group (P < 0.0001).

Key words: Endometriosis, Polymorphism, Infertility, Estrogen receptor.