Y.H. Pang, C.Z. Lei, C.L. Zhang, X.Y. Lan, S. Shao, X.M. Gao, J.Q. Wang, H. Chen
Published: March 01, 2011
Genet. Mol. Res. 10(1): 359-367
DOI: https://doi.org/10.4238/vol10-1gmr1041

Cite this Article:
Y.H. Pang, C.Z. Lei, C.L. Zhang, X.Y. Lan, S. Shao, X.M. Gao, J.Q. Wang, H. Chen (2011). Lack of association of single nucleotide polymorphisms of the bovine Flt-1 gene with growth traits in Chinese cattle breeds. Genet. Mol. Res. 10(1): 359-367. https://doi.org/10.4238/vol10-1gmr1041

About the Authors
Y.H. Pang, C.Z. Lei, C.L. Zhang, X.Y. Lan, S. Shao, X.M. Gao, J.Q. Wang, H. Chen
Corresponding Author: H. Chen
Email: chenhong1212@263.net

ABSTRACT

We analyzed 20 exons, with their intron-exon boundaries, of the bovine Flt-1 gene, using a strategy combining PCR amplification and single-strand conformational polymorphism analysis (PCR-SSCP), followed by nucleotide sequence analysis, in 675 cattle. We then looked for associations between polymorphisms and growth traits. Twelve novel SNPs (ss#184956516, ss#184956517, ss#184956518, ss#184956519, ss#251343993, ss#251343994, ss#251343995, ss#251343996, ss#251343997, ss#251343998, ss#251343999, and ss#251344000) were detected in the bovine Flt-1 gene in all three breeds. We observed no significant associations between these polymorphisms and birth weight, body weight and average daily gain during different growth periods (6, 12, 18, and 24 months old) (P > 0.05), or in body height, body length, heart girth, or height at the hip in Nanyang cattle breeds.

Key words: Single nucleotide polymorphism, Chinese cattle, Flt-1 gene.