H.A. Marquez-Santacruz, R. Hernandez-Leon, M.C. Orozco-Mosqueda, I. Velazquez-Sepulveda and G. Santoyo
Published December 7, 2010
Genet. Mol. Res. 9 (4): 2372-2380 (2010)
DOI 10.4238/vol9-4gmr921
About the Authors
H.A. Marquez-Santacruz, R. Hernandez-Leon, M.C. Orozco-Mosqueda, I. Velazquez-Sepulveda and G. Santoyo
Corresponding author:
G. Santoyo
E-mail: gustavo_santoyo@yahoo.com / gsantoyo@umich.mx
ABSTRACT
Endophytic bacterial diversity was estimated in Mexican husk tomato plant roots by amplified rDNA restriction analysis and sequence homology comparison of the 16S rDNA genes. Sixteen operational taxonomic units from the 16S rDNA root library were identified based on sequence analysis, including the classes Gammaproteobacteria, Betaproteobacteria, Actinobacteria, and Bacilli. The predominant genera were Stenotrophomonas (21.9%), Microbacterium (17.1%), Burkholderia (14.3%), Bacillus (14.3%), and Pseudomonas (10.5%). In a 16S rDNA gene library of the same plant species’ rhizosphere, only common soil bacteria, including Stenotrophomonas, Burkholderia, Bacillus, and Pseudomonas, were detected. We suggest that the endophytic bacterial diversity within the roots of Mexican husk tomato plants is a subset of the rhizosphere bacterial population, dominated by a few genera.
Key words: Endophytes; Bacterial diversity; Rhizosphere; Mexican husk tomato plants.