L.V. Medeiros, D.B. Maciel, V.V. Medeiros, L.M.Houllou Kido, N.T. Oliveira
Published April 13, 2010
Genet. Mol. Res. 9 (2): 661-673 (2010)
DOI 10.4238/vol9-2gmr725

About the Authors
L.V. Medeiros, D.B. Maciel, V.V. Medeiros, L.M.Houllou Kido, N.T. Oliveira

Corresponding author
L.V. Medeiros
Email: lilivmedeiros@yahoo.com.br

ABSTRACT

Colletotrichum gloeosporioides is an important pathogen for a great number of economically important crops. During the necrotrophic phase of infection by Colletotrichum spp, the degradative enzymes of plant cell walls, such as pectate lyase, clearly increase. A gene pelB that expresses a pectate lyase was identified in isolates of C. gloeosporioides in avocado pathogens. Various molecular studies have identified a kind of specialization of C. gloeosporioides isolates with specific hosts; however, there have been no studies of this gene in isolates from hosts other than avocado. The same is true for other species of Colletotrichum. We examined genetic variability in order to design primers that would amplify pelB gene fragments and compared the products of this amplification in C. gloeosporioides isolates from different hosts. Genetic variability was assessed using ISSR primers; the resultant data were grouped based on the UPGMA clustering method. Primers for the pelB gene were designed from selected GenBank sequences using the Primer 3 program at an annealing temperature of 60°C and product amplification of nearly 600 bp. The ISSR primers were efficient in demonstrating the genetic variability of the Colletotrichum isolates and in distinguishing C. gloeosporioides, C. acutatum and C. sublineolum species. The gene pelB was found in C. gloeosporioides, C. acutatum and C. sublineolum. Amplified restriction fragments using MspI did not reveal differences in pelB gene structure in isolates from the three different host species that we investigated.

Key words: Colletotrichum, CgInt, ISSR, pelB gene, Pectate lyase.