L.Q. Han, H.J. Li, Y.Y. Wang, H.S. Zhu, L.F. Wang, Y.J. Guo, W.F. Lu, Y.L. Wang and G.Y. Yang
Published June 29, 2010
Genet. Mol. Res. 9 (2): 1250-1257 (2010)
DOI 10.4238/vol9-2gmr814

About the authors
L.Q. Han, H.J. Li, Y.Y. Wang, H.S. Zhu, L.F. Wang, Y.J. Guo, W.F. Lu, Y.L. Wang and G.Y. Yang

Corresponding author
G.Y. Yang
E-mail: mrswx@yahoo.cn

ABSTRACT

The functions of distinct isoforms of solute carrier family 27 transporters (SLC27A1-6), acetyl-CoA carboxylase (ACACA, ACACB), stearoyl-CoA desaturase (SCD1-4), fatty acid desaturase (FADS1-3), LPIN (LPIN1-3), insulin-induced gene (INSIG1, 2), and peroxisome proliferator-activated receptor gamma coactivator1 (PPARGC1A, B) were studied in the mouse mammary gland from pregnancy to lactation. The relative mRNA abundance and percent change in real-time PCR were determined. mRNA expression of SLC27A3 and SLC27A4 was 37- and 1.4-fold more upregulated at 12 days of lactation, respectively (P < 0.01). Transcripts of SCD isoforms were the most abundant, accounting for 59% of all genes measured, and PPARGC1 isoforms were the least (0.06% of all genes measured). The mRNA abundance from ACC, FADS and LPIN accounted for 29, 9 and 2.6%, respectively. INSIG1 mRNA expression was 32-fold more upregulated (P < 0.05), while PPARGC1B was 0.18-fold downregulated at 18 days of lactation (P < 0.01). We concluded thatmRNA abundance and expression of these isoforms are affected by the stage of lactation.

 Key words: Isoform; Lactation; Lipogenic gene; Mouse mammary gland; Quantitative real-time PCR