S. Nawaz, F.A. Khan, S. Tabasum, M.Z. Iqbal and A. Saeed
Published June 1, 2010
Genet. Mol. Res. 9 (2): 1011-1022 (2010)
DOI 10.4238/vol9-2gmr795

About the authors
S. Nawaz, F.A. Khan, S. Tabasum, M.Z. Iqbal and A. Saeed

Corresponding author
A. Saeed
E-mail: drasifpbg@gmail.com

ABSTRACT

Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. Genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. The shortest genetic distance of 0.60 was seen between genotypes US-804 and US-130. These two genotypes differed from each other only in 10 bands, with 20 primers. The most dissimilar of the accessions were CP-77-400 and US-133, with a genetic distance of 1.11. SSR fingerprints can help sugarcane breeders to clarify the genetic pedigree of commercial sugarcane varieties and evaluate the efficiency of breeding methods.

Key words: DNA marker; SSR; Genetic distance; Fingerprinting; Saccharum