Y. Erturk, S. Ercisli, D. Maghradze, E. Orhan, G. Agar
Published: October 13, 2009
Genet. Mol. Res. 8 (4) : 1238-1244
DOI: https://doi.org/10.4238/vol8-4gmr641
Cite this Article:
Y. Erturk, S. Ercisli, D. Maghradze, E. Orhan, G. Agar (2009). An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers. Genet. Mol. Res. 8(4): 1238-1244. https://doi.org/10.4238/vol8-4gmr641
About the Authors
Y. Erturk, S. Ercisli, D. Maghradze, E. Orhan, G. Agar
Corresponding author
S. Ercisli
E-mail: sercisli@hotmail.com
ABSTRACT
Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic. A UPGMA dendrogram clearly divided the genotypes into four groups; we concluded that RAPD analysis can be used for examining genetic relatedness among blackthorn genotypes.
Key words: Genetic diversity, Random amplified polymorphic DNA, Blackthorn, Prunus spinosa.