F.A. Martins, P.C.S. Carneiro, C.T. Guimarães, J.V. Magalhães, J.E.S. Carneiro, C.D. Cruz
Published: March 17, 2009
Genet. Mol. Res. 8 (1) : 319-327
DOI: https://doi.org/10.4238/vol8-1gmr585
Cite this Article:
F.A. Martins, P.C.S. Carneiro, C.T. Guimarães, J.V. Magalhães, J.E.S. Carneiro, C.D. Cruz (2009). Distinction between plant samples according to allele dosage by semiquantitative polymerase chain reaction. Genet. Mol. Res. 8(1): 319-327. https://doi.org/10.4238/vol8-1gmr585
About the Authors
F.A. Martins, P.C.S. Carneiro, C.T. Guimarães, J.V. Magalhães, J.E.S. Carneiro, C.D. Cruz
Corresponding author
F.A. Martins
E-mail: franufv@hotmail.com
ABSTRACT
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that a heterozygote (Aa) has AAa or Aaa endosperm, when the female genitor donates the A or a allele, respectively. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy.
The lack of informativity of samples from heterozygotic individuals is one of the hindrances in the mapping of quantitative trait loci of outbred populations, since it is not normally possible to identify the origin of each allele. One way to include these individuals in analyses would be to genotype their endosperm, considering that a heterozygote (Aa) has AAa or Aaa endosperm, when the female genitor donates the A or a allele, respectively. We used semiquantitative polymerase chain reaction to determine allele dosages in DNA mixtures, by simulating the observed conditions for endospermic tissue. Semiquantitative polymerase chain reaction on agarose gels, along with regression analysis, allowed differentiation of the samples according to the amount of DNA. This type of information will help decrease the number of non-informative individuals in quantitative trait locus mapping of outbred populations, thereby increasing mapping accuracy.
Key words: Allele dosage, Endosperm constitution, Maize, Semiquantitative polymerase chain reaction.