L.R.S. Moreira, A.L. Miranda-Vilela, I.C.R. Silva, A.K. Akimoto, M.N. Klautau-Guimarães, C.K. Grisolia
Published: March 10, 2009
Genet. Mol. Res. 8 (1) : 284-290
DOI: https://doi.org/10.4238/vol8-1gmr569
Cite this Article:
L.R.S. Moreira, A.L. Miranda-Vilela, I.C.R. Silva, A.K. Akimoto, M.N. Klautau-Guimarães, C.K. Grisolia (2009). Antioxidant effect of haptoglobin phenotypes against DNA damage induced by hydrogen peroxide in human leukocytes. Genet. Mol. Res. 8(1): 284-290. https://doi.org/10.4238/vol8-1gmr569
About the Authors
L.R.S. Moreira, A.L. Miranda-Vilela, I.C.R. Silva, A.K. Akimoto, M.N. Klautau-Guimarães, C.K. Grisolia
Corresponding author
L.R.S. Moreira
E-mail: leonorarsm@gmail.com
ABSTRACT
Human haptoglobin is classified into three major phenotypes: Hp1-1, Hp2-1 and Hp2-2; there are two autosomal alleles Hp*1 and Hp*2, and the Hp*1 allele has two subtypes, Hp*1F and Hp*1S. Haptoglobin acts as an antioxidant, preventing hemoglobin-driven oxidative damage. We used the comet assay to examine oxidative damage to DNA induced by hydrogen peroxide in human leukocytes; we also looked for differences in the antioxidant capacity of haptoglobin subtypes. Haptoglobin genotypes were determined through allele-specific polymerase chain reaction, visualized on a polyacrylamide gel. The Hp1-1 genotype had the least DNA damage; this indicates that Hp alleles differ in their protective effects against oxidative damage. Among Hp*1 alleles, Hp*1F was the most protective.
Human haptoglobin is classified into three major phenotypes: Hp1-1, Hp2-1 and Hp2-2; there are two autosomal alleles Hp*1 and Hp*2, and the Hp*1 allele has two subtypes, Hp*1F and Hp*1S. Haptoglobin acts as an antioxidant, preventing hemoglobin-driven oxidative damage. We used the comet assay to examine oxidative damage to DNA induced by hydrogen peroxide in human leukocytes; we also looked for differences in the antioxidant capacity of haptoglobin subtypes. Haptoglobin genotypes were determined through allele-specific polymerase chain reaction, visualized on a polyacrylamide gel. The Hp1-1 genotype had the least DNA damage; this indicates that Hp alleles differ in their protective effects against oxidative damage. Among Hp*1 alleles, Hp*1F was the most protective.
Key words: Antioxidant, Comet assay, haptoglobin polymorphism, hydrogen peroxide.