J.F. Barbosa, J.P. Bravo, D.B. Zanatta, J.L.C. Silva, M.A. Fernandez
Published: February 25, 2009
Genet. Mol. Res. 8 (1) : 197-206
DOI: https://doi.org/10.4238/vol8-1gmr543
Cite this Article:
J.F. Barbosa, J.P. Bravo, D.B. Zanatta, J.L.C. Silva, M.A. Fernandez (2009). Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae). Genet. Mol. Res. 8(1): 197-206. https://doi.org/10.4238/vol8-1gmr543
About the Authors
J.F. Barbosa, J.P. Bravo, D.B. Zanatta, J.L.C. Silva, M.A. Fernandez
Corresponding author
M.A. Fernandez
E-mail: aparecidafernandez@gmail.com
ABSTRACT
Conformation-sensitive gel electrophoresis is a useful method for identifying allele polymorphism; it provides co-dominant molecular markers. Using this method, we identified genetic variability in the third intron of the fibroin light chain gene, fib-L, in six Bombyx mori strains. Only Chinese C21A strain did not demonstrate allelic alterations, showing only homoduplex DNA molecules. We found distinct heteroduplex profiles in the Japanese HAA, M12B and M19-2 and the Chinese C25B and C24-2 strains. Analysis with restriction endonuclease fingerprinting conformation-sensitive gel electrophoresis demonstrated the potential of this method for the identification of allelic variability in B. mori; this was confirmed by cloning and sequencing the different alleles. The main alteration was a 12-bp deletion in two alleles of the C24-2 strain and one allele of the HAA strain; this deletion results in specific heteroduplex DNA molecule profiles.
Conformation-sensitive gel electrophoresis is a useful method for identifying allele polymorphism; it provides co-dominant molecular markers. Using this method, we identified genetic variability in the third intron of the fibroin light chain gene, fib-L, in six Bombyx mori strains. Only Chinese C21A strain did not demonstrate allelic alterations, showing only homoduplex DNA molecules. We found distinct heteroduplex profiles in the Japanese HAA, M12B and M19-2 and the Chinese C25B and C24-2 strains. Analysis with restriction endonuclease fingerprinting conformation-sensitive gel electrophoresis demonstrated the potential of this method for the identification of allelic variability in B. mori; this was confirmed by cloning and sequencing the different alleles. The main alteration was a 12-bp deletion in two alleles of the C24-2 strain and one allele of the HAA strain; this deletion results in specific heteroduplex DNA molecule profiles.
Key words: Bombyx mori, Silkworm, Conformation-sensitive gel electrophoresis, Allelic variability, Fibroin light chain.