S.R.C. Campos, C. Trindade, O.P. Ferraz, D.N.S. Giovanni, A.A. Lima, H.V.A. Caetano, R.F. Carvalho, E.H. Birgel Jr., M.L.Z. Dagli, E. Mori, P.E. Brandão, L.J. Richtzenhain, W. Beçak, R.C. Stocco
Published: October 21, 2008
Genet. Mol. Res. 7 (4) : 1119-1126
DOI: https://doi.org/10.4238/vol7-4gmr498

Cite this Article:
S.R.C. Campos, C. Trindade, O.P. Ferraz, D.N.S. Giovanni, A.A. Lima, H.V.A. Caetano, R.F. Carvalho, E.H. Birgel, M.L.Z. Dagli, E. Mori, P.E. Brandão, L.J. Richtzenhain, W. Beçak, R.C. Stocco (2008). Can established cultured papilloma cells harbor bovine papillomavirus?. Genet. Mol. Res. 7(4): 1119-1126. https://doi.org/10.4238/vol7-4gmr498

About the Authors
S.R.C. Campos, C. Trindade, O.P. Ferraz, D.N.S. Giovanni, A.A. Lima, H.V.A. Caetano, R.F. Carvalho, E.H. Birgel Jr., M.L.Z. Dagli, E. Mori, P.E. Brandão, L.J. Richtzenhain, W. Beçak, R.C. Stocco

Corresponding author
R.C. Stocco
E-mail: ritastocco@butantan.gov.br

ABSTRACT

Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA sequences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the simultaneous presence of different BPV DNA sequences, comparing the original lesion to different passages of the cell cultures and to peripheral blood. BPV 1, 2 and 4 DNA sequences were found in lesion samples, and respective cell cultures and peripheral blood, supporting our previous hypothesis of the possible activity of these sequences in different samples and now also showing how they can be maintained in different passages of cell cultures.

Key words: Papillomavirus, Bovine papilloma virus, primary cell cultures, wart, cutaneous papillomatosis.