H.A. Khan, I.A. Arif, A.A. Al Homaidan, A.H. Al Farhan
Published: December 16, 2008
Genet. Mol. Res. 7 (4) : 1392-1397
DOI: https://doi.org/10.4238/vol7-4gmr514
Cite this Article:
H.A. Khan, I.A. Arif, A.A.Al Homaidan, A.H.Al Farhan (2008). Application of 16S rRNA, cytochrome b and control region sequences for understanding the phylogenetic relationships in Oryx species. Genet. Mol. Res. 7(4): 1392-1397. https://doi.org/10.4238/vol7-4gmr514
About the Authors
H.A. Khan, I.A. Arif, A.A. Al Homaidan, A.H. Al Farhan
Corresponding author
H.A. Khan
E-mail: khan_haseeb@yahoo.com
ABSTRACT
The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the topologies of respective phylogenetic trees were compared. All these markers clearly differentiated the genus Addax from Oryx. However, for species-level grouping, while 16S rRNA and cytochrome b produced similar phylogeny (SHO grouped with PO), the control region grouped SHO with AO. Further studies are warranted to generate more sequencing data, apply multiple bioinformatics tools and to include relevant nuclear markers for phylogenetic analysis of Oryx species.
Key words: Conservation, Mitochondrial DNA, Oryx, Endangered species, Phylogenetic relationship.